高效液相色谱法测定袋泡茶叶中的黄曲霉毒素

Determination of aflatoxins in tea bags by high performance liquid chromatography

目的建立袋泡茶叶中4种黄曲霉毒素G1,B1，G2，B2的高效液相色谱测定方法。方法袋泡茶叶经乙腊水溶液超声提取30min，多功能固相萃取柱净化，将一定量的提取液转移至衍生瓶中，氮吹仪吹干，用正己皖和三氟乙酸在 40℃条件下衍生15min后氮吹仪吹干，乙脯水溶液定容，采用C18柱分离，甲醇水（45 ＋ 55）溶液进行洗脱，荧光检测器 检测。结果在最适条件下，黄曲霉毒素G1，B1，G2，B2的分离效果好，在2个不同添加水平下，样品的平均回收率在 729毛-82.3%之间，RSD在3.3% -4.5%之间，线性关系良好，r2〉0.9990 ，四种黄曲霉毒素G1，B1,G2，B2的检出限分别为0.10μg/kg，0.002μg/kg，0.005μg/kg，0.017μg/kg。结论本文的方法灵敏度好、简单、快速、定量准确，适用于袋泡茶叶中黄曲霉毒素G＂吭，G2，B2的同时测定。

Objective To establish a method for determination of aflatoxin C. , B, , Gz, Bz in tea bags by high performance liquid chromatography. Methods Samples were extracted ultrasonically with acetonitrile - water solution for 30 minutes, purified with multifunctional SPE column, dried by nitrogen blowing concentrator in a bottle, derived with n - hexane and trifluoroacetic acid at 40 ＂C for 15 min, then dried by nitrogen blowing concentrator again for acetonitrile constant volume. The analytes were separated by C18 column, eluted with methanol - water （45 ＋ 55） solution, and finally detected by fluorescence detector. Results Under optimum conditions, the separation of aflatoxin Gj, BJ , Gz, Bz was good, and the average recoveries were between 72% -82.3% at two adding levels. RSD was from 3.3% to 4.5%, all had good linear relationship, r2 〉0.9990. The detection limits of aflatoxin Gj, Bj, Gz, B, were 0.10 μg/kg, 0.002 μg/kg, 0.005 μg/kg, 0.017μg/kg, respectively. Conclusion The method is sensitive, simple, rapid and accurate. It is suitable for the simultaneous determination of aflatoxin Gj , BJ , Gz, B, in tea bags.