腹泻病人便标本中单增李斯特菌的分离与检测

Isolation and identification of Listeria monocytogenes in feces samples from diarrhea patients

目的了解单核细胞增生李斯特菌在腹泻病人便标本中的携带及感染状况,探讨食源性单增李斯特菌与临床病例之间的关系,为由其引起的食物中毒病原学及食品中单增李斯特菌污染状况调查提供数据参考。方法采集本辖区医院肠道门诊腹泻患者病例便标本,分别采用两种不同的增菌液和培养基进行分离培养,使用梅里埃VITEK 2 Compact30全自动细菌生化分析仪,进行单增李斯特菌的鉴定。结果在410例腹泻病人便标本中,检出1株单增李斯特菌,检出率0.24%。结论腹泻病人便标本中可以检出单增李斯特菌,为了提高感染人群标本中单增李斯特菌的检出率,需要进一步优化分离培养条件。

Objective To understand the infection situation of Listeria monocytogenes in diarrhea patients and to discuss the re- lationship between Listeria monocytogenes and clinical diarrhea patients, so as to provide data reference for etiological investiga- tion on food poisoning and Listeria monocytogenes pollution in food. Methods Fecal samples were collected from diarrhea pa- tients fo~ isolation and culture of Listeria monocytogenes using two different enrichment broth and media, then VITEK2 Com- pact30 automatic biochemical analyzer was adopted for Listeria nwnocytogenes identification. Results Only one Lister＆ monocy- togenes strain was isolated from 410 diarrhea feces samples, the positive rate was 0.24%. Conclusion Listeria monocytogenes can be isolated from fecal samples of diarrhea patients. The bacteria isolation procedure should be optimized to improve the de- tection rate of Listeria monocytogenes in population who were infected.