平喘固本膏方对COPD稳定期大鼠血清CRP、TNF-α、IL-6、IL-8及肺功能的影响

Effect of Pingchuanguben Decoction on CRP,TNF-α,IL-6,IL-8 and Lung Function. of Rat Models with COPD in the Stable Period

目的研究平喘固本膏方对COPD稳定期大鼠血清CRP、TNF-α、IL-6、IL-8及肺功能的影响。方法采用SD大鼠制作COPD稳定期模型,并用平喘固本膏方对其进行干预,疗程为1个月,对照组采用茶碱缓释片灌胃,治疗后进行肺功能检测,并取血清,以双抗体夹心ABC-ELISA法检测CRP、TNF-α、IL-6和IL-8。结果各治疗组IL-6、IL-8水平均低于对照组(P<0.05),仅高剂量膏方组TNF-α水平低于对照组(P>0.05),对照组与各膏方组CRP水平相近(P>0.05)。在跨肺压及潮气量方面,膏方各剂量与氨茶碱作用相近(P>0.05),在气道流速方面,膏方中高剂量组优于氨茶碱组(P<0.05)。结论平喘固本汤膏方可降低COPD稳定期血清CRP、TNF-α、IL-6和IL-8水平,但对肺功能的影响不明显。

Objective： To study the effect of Pingchuanguben Decoction on rat models of COPD in the stable pe- riod on CRP,TNF-α,IL-6,IL-8 and lung function. Methods： The model of COPD in the stable period was made with SD rats and treated with Pingchuanguben Decoction for 1 month. The control group was treated with amino- phylline sustained-release tablets. After treatment,lung function was tested and then serum was taken in which CRP,TNF-α,IL-6,IL-8 were tested. Results： IL-6 and IL-8 levels in treatment groups were lower remarkably than those in control group （P〈 0.05）. Only TNF-α level in the high dose treatment group was lower than that in control group （P〉 0.05）. As for CRP, transpulmonary pressure and tidal volume,there were no significant differ- ences between in treatment groups and control group （P〉 0.05）. Airway flow of medium and high dose treatment group was lower obviously than that in control group （P〈 0.05）. Conclusion： Pingchuanguben Decoction can de- crease serum levels of CRP,TNF-α,IL-6,IL-8 in COPD of the stable period.