CD44重组表达抑制鼻咽癌6-10B细胞增殖

Expression of recombinant CD44 inhibits the proliferation of nasopharyngeal carcinoma 6-10B cell line

目的:探索CD44v10剪接变异体对鼻咽癌细胞增殖的生物学作用。方法:将CD44v10基因编码序列重组,构建pIRESneo3-CD44v10重组质粒表达载体,重组表达载体扩增后采用脂质体转入鼻咽癌6-10B细胞系,G418筛选稳定转染细胞株;RT-PCR和蛋白印迹鉴定CD44v10重组表达6-10B细胞株;MTT法分析细胞增殖。结果:获得了CD44v10稳定重组表达的6-10B细胞株;CD44v10表达抑制6-10B细胞增殖。结论:CD44v10表达对鼻咽癌细胞的恶性增殖可能具有抑制作用。

Objective： To explore the biological function of splice variants of CD44v10 on the proliferation of nasopharyngeal carcinoma cells. Methods： The sequence of CD44v10 gene was constructed with pIRESneo3 plasmid expression vector to build recombinant pIRESneo3-CD44v10, the recombinant expression vector was amplified and transfected into nasopharyngeal carcinoma 6-10B cell line by liposome, the stably transfected cell strains were screened by G418, CD44v10 recombinant expression 6-10B cells were detected by RT-PCR and western blot. Cell proliferation was tested by MTT experiment. Results： The stable CD44v10 expression 6-10B cells were gotten. Expression of CD44v10 inhibited proliferation of 6-10B cells. Conclusion. The expression of CD44v10 may inhibit the malignant proliferation of nasopharyngeal carcinoma cells.