催化体系共振散射光谱分析纳米金含量

The analysis of nanogold content by resonance light scattering spectral of catalytic system

目的：建立纳米金纳米催化共振散射光谱新方法.方法：在13.3 μg/ml氯金酸、4.7mmol/L盐酸、2.40 mg/ml溴代十六烷基三甲铵（CTMAB）和25.0 mg/ml维生素C条件下,纳米金催化维生素C还原生成的单质Au在纳米金微粒表面沉积形成较大粒径的金纳米微粒.结果：体系在600 nm处有一共振散射峰,随着纳米金浓度的增大,催化作用加强,600 nm处共振散射峰升高,其升高值与纳米金浓度在0.480～96.0 ng/ml范围内存在良好的线性关系,回归方程为△I=8.559C＋14.48,相关系数为0.9972,检出限为0.056 ng/ml.结论：纳米催化共振散射光谱能较好地定量分析纳米金的含量.

Objective. To set up a new nano-catalytic resonance light scattering spectral method of nanogold. Methods： Under the condition of 13.3 μg/ml chloroauric acid, 4. 7 mmol/Lchlorhydric acid, 2.40 mg/ml CTMAB and 25.0 mg/ml Vatmin C, elemental Au by the reduction of nanogold catalytic Vitamin C forms sedimentarily larger particle size of gold nanoparticles on the surface of nanogold particles. Results： There exists a resonance scattering peak in the 600 nm of th＇e nanogold catalytic system. With the increase of the nanogold concentration and the enhancement of the catalytic effect, the resonance scattering peak elevates. There is a better linear relation of the elevation value with the nanogold concentration in the concentration scope between 0. 480 and 96. 0 ng/ml, and the regression equation is △I=8. 559C＋14.48, the correlation coefficient is 0.9.972 and the detection limit is 0.056 ng/ml. Conclusion. Nano-catalytic resonance light scattering spectral method is suitable for the quantitative analysis of the nanogold concentration.