狂犬病病毒感染对宿主miR-29a和miR-124表达的影响及其靶基因分析

Effect of rabies virus infection on host miR-29a and miR-124 expression and bioinformatics target gene analysis

依据本实验室前期狂犬病病毒(RABV)感染小鼠原代神经元microRNA(miRNA)表达谱数据,选取表达显著上调的miR-29a和下调miR-124为研究对象,采用实时荧光定量PCR技术检测目的 miRNA在RABV感染前后的小鼠原代神经元和小鼠海马组织内的表达变化。运用TargetScan数据库预测目的 miRNA潜在的靶基因,并对靶基因进行了GO(Gene Ontology)注释描述、GO注释富集分析和信号通路富集分析,富集结果呈现出数个与神经功能相关的GO注释和信号通路。本研究结果提示miR-29a和miR-124可能参与了RABV感染所致的宿主神经功能异常。

The up-regulated miR-29a and down-regulated miR-124 were selected from our previous data of miRNA expression pattern induced by rabies virus （RABV） infection in primary neuron of mice. The expression levels of miR-29a and miR-124 were validated in primary neurons and hipp- ocampal tissues of mice infected with RABV by quantitative real-time PCR, respectively. Next, po- tential target genes of miR-29a and miR-124 were predicted by TargetScan database. Further- more,Gene Ontology （GO） and biological pathway enrichment analysis showed several neuronal function-related GO terms and intracellular biological pathways, which indicated that miR-29a and miR-124 may be involved in neuronal dysfunction caused by RABV.