延边黄牛TG基因多态性与胴体和肉质性状相关性分析

Association between the polymorphism of TG gene and meat quality traits in Yanbian yellow cattles

选取105头延边黄牛为研究对象,利用克隆测序的方法寻找延边黄牛甲状腺球蛋白(thyroglobulin,TG)基因多态性,采用PCR-RLFP方法进行多态性检测,并与胴体和肉质性状进行了关联分析。结果表明,在TG基因第48外显子上发现C218T和A430G2个突变位点,其中C218T位点的不同基因型与大理石花纹存在显著相关,CC基因型个体大理石花纹等级显著高于CT型个体(P<0.05)。A430G位点的不同基因型与宰前活重和眼肌面积存在显著相关,GG基因型个体的宰前活重和眼肌面积显著高于AA型和AG型个体(P<0.05)。其他性状在2个位点的不同基因型间差异不显著。因此,C218T和A430G位点可能是影响延边黄牛胴体和肉质性状的分子标记。

The aim was to （TG） on the carcass and investigate the effect of the genetic polymorphisms of thyroglobulin gene meat quality traits in 105 Yanbian yellow tattles. The SNPs were detec- ted by cloning and sequencing, confirmed by technique of PCR-RFLP, and analyzed the association of polymorphisms of TG gene with carcass and meat quality traits. The results showed that there were C218T and A430G two mutations in the exon 48 in TG gene of Yanbian yellow cattle. The association analysis showed that marbling score with CC genotype in C218T locus was significant- ly higher than that of CT genotype（P〈0.05）. A430G locus polymorphism significantly associated with live weight and loin muscle area. Individuals with genotype GG had significantly larger live weight and loin muscle area than those with AA and AG genotype（P（0.05）. In a word,C218T and A430G SNPs might be a good DNA marker to be used in MAS for carcass and meat quality traits.