摘要
通过正交试验确定最佳的粗提工艺,并将林蛙输卵管水提液先后通过盐析,透析,阴离子交换层析、阳离子交换层析和葡聚糖凝胶过滤层析,获得了一种分子量大小约为66 kD的单一蛋白,高碘酸-Schiff试剂法鉴定其为糖蛋白。经HPLC分析,纯度为97.2%,将其命名为ROGP-Ⅲ,经PNGase F糖苷肽酶处理后推测其糖含量约为17%。
To study the function of the single glycoprotein in Rana chensinensis, this research determined the best coarse extraction process by orthogonal test, then purified a 66 kD single protein by salting out, dialysis, ion-exchange column chromatography and gel filtration chromatography. The purified protein identified as glycoprotein by PAS test. By HPLC identification, its purity is 97.2%, named as ROGP-Ⅲ, after enzymolysis by PNGase F, it speculated that the polysaccharide content is about 17%.
出处
《生物技术通报》
CAS
CSCD
北大核心
2014年第2期102-106,共5页
Biotechnology Bulletin
基金
国家自然科学基金项目(30671536)
关键词
林蛙油
糖蛋白
分离
纯化
鉴定
Oviducts Rana Glycoprotein Separation Purification Identification