摘要
利用小白鼠免疫和噬菌体展示技术构建了噬菌体展示抗体文库,从文库中筛选获得了一株抗克伦特罗单克隆抗体。该抗体的氨基酸序列尚未在世界基因数据库中登录,是一个新的抗体。该抗体对于游离克伦特罗的半抑制率IC50为0.602μg/mL。该噬菌体展示的抗体片段可以用于竞争法检测克伦特罗的含量,其能够检测出的最低浓度为15.6 ng/mL,具有较大的实用价值。
An anti-clenbuterol antibody was developed by immunization of mouse and construction of a phage displaying antibody library. The antibody has a novel sequence and the utilization of gene segments in germline was also identified. With this antibody, a competitive assay was performed and gave a limit of detection of 15.6 ng/mL. The IC 50 of phage-antibody with free clenbuterol against immobilized bovine serum albumin conjugated clenbuterol was 0.602 μg/mL. All the results suggested that the antibody is useful in practical applications.
出处
《生物技术通报》
CAS
CSCD
北大核心
2014年第2期136-142,共7页
Biotechnology Bulletin
基金
潍坊科技学院校级课题(博士基金)(W13K007)
关键词
克伦特罗
抗体
噬菌体展示
免疫检测
Clenbuterol Monoclonal antibody Phage display Detection
