摘要
CGA-N46是人嗜铬粒蛋白N端具有抗真菌活性的多肽片段。采用MTT法检测CGA-N46对克柔念珠菌生长的抑制作用,以β-1,3-葡聚糖酶活性检测克柔念珠菌细胞壁的完整性,采用PI检测克柔念珠菌细胞膜通透性,用罗丹明123检测线粒体膜电位。试验结果证实,0.5 mg/mL的CGA-N46能够明显抑制克柔念珠菌的细胞增殖能力;对其细胞壁的完整性及细胞膜的通透性没有影响;CGA-N46能明显降低线粒体膜电位。推测CGA-N46可能通过影响线粒体膜电位来抑制克柔念珠菌的生长。
CGA-N46 is an N terminal derived peptide of human Chromogranin A(CGA) that has anti-fungal activities. The growth inhibition effect on Candida krusei was measured with MTT assay. The cell wall integrity of Candida krusei was examined by monitoring β-1,3-glucanase activity. The effect of CGA-N46 on the permeability of Candida krusei membrane was examined using Propidium iodide detection. The effect of CGA-N46 on mitochondrial membrane potential was evaluated through Rh-123 assay. The results of showed 0.5 mg/mL CGA-N46 could significantly inhibit the proliferation of the C. krusei cells. CGA-N46 had no obvious effect on the β-1, 3-glucanase activity. There was no significant influence on the permeability of C. krusei cell membrane; CGA-N46 could significantly decrease the mitochondrial membrane potential. A conclusion can therefore be made that CGA-N46 may inhibit the growth of Candidas by affecting the mitochondrial membrane potential.
出处
《生物技术通报》
CAS
CSCD
北大核心
2014年第2期148-152,共5页
Biotechnology Bulletin
基金
国家自然科学基金项目(31071922)
河南省重点科技攻关计划项目(112102310325)
河南工业大学科学研究基金研究生教育创新计划项目(12YJCX51)