胰头夹闭法致大鼠重症急性胰腺炎相关性肾损伤动物模型的建立

To establish rat models of severe acute pancreatitis with renal injury by obstructing the head of pancreas

目的通过暂时夹闭胰头部的方法建立一种新型大鼠重症急性胰腺炎（SAP）相关性肾损伤的动物模型。方法将健康SD大鼠144只随机分为对照组、假手术组、模型组，每组48只。模型组开腹后钝性分离胰腺周围韧带至胰头部，无创血管钳夹闭胰头3h后松开。在造模完成后每组分别于0、6、12、18、24、30、36、48h各时间点随机取6只大鼠检测血淀粉酶（AMY）、肌酐（cr）、尿素氮（BUN），再分别取胰腺及肾组织行病理组织学观察。结果模型组大鼠血淀粉酶活性及12h后肌酐、尿素氮浓度较对照组和假手术组明显升高（P〈0．05）。病理组织学观察可见造模完成后随时间进程胰腺和肾损伤呈加重表现。结论通过夹闭胰头部的方法成功建立了一种新型大鼠SAP相关性。肾损伤动物模型，为SAP相关性肾损伤的发病机制及药物干预研究提供了一种较理想的动物模型。

Objective To establish rat models of severe acute pancreatitis （SAP）with renal injury by obstructing the head of pancreas. Methods A total of 144 rats were randomly divided into control group（ n =48 ）, sham - operated group （ n = 48 ）, model group （ n = 48 ）. The pancreas and pancreatic tail - segment was dissociated and the head of pancreas was occluded in the rats to establish the models, blood vessel forceps was loosed after three hours. All the rats were sacrificed at 0, 6, 12, 18, 24, 36 and 48 h after modeling. The level of serum amylase（AMY） , creatinine（Cr） , blood urea nitrogen（BUN） were detected and pathological change of pancreas and kidneys were observed under light microscope. Results The concentrations of serum amylase, Cr, BUN were significantly higher in model group than in other groups （P 〈 0.05 ）. The tissue damage of pancreas and kidneys became more serious after the establishment of rat models. Conclusion The models show a typical severe acute pancreatitis with renal injury by obstructing the head of pancreas. It is an ideal and clinically relevant animal model for study of nosogenesis and therapeutical effect of severe acute pancreatitis with renal injury.