生物芯片技术用于同时测定乳与乳粉中β-兴奋剂类残留量

Determination of β-Agonists Multipleresidues with protein biochip of in milk and milk powder

利用生物芯片β-兴奋剂类组合,选用其中β-兴奋剂类、群勃龙、皮质类固醇三个组分同时定量测定其残留量.考察了该生物芯片用于定量测定乳β-兴奋剂类残留的准确性、精密度等是否能满足检测的要求.本方法线性范围宽为0～5 ng/mL；β-兴奋剂类、群勃龙、皮质类固醇最低检出限分别为0.1、0.5、0.2 μg/kg.2个水平的加标（2倍检出限与5倍检出限添加量）回收实验；其回收率在99%130％间;其中加入后者回收率优于前者;通过对2倍与5倍加标样品的精密度测定结果,其变异系数在14％以内.通过对阴性样品测定全部低于相应的检出限,质控样品测定结果为参考值的105％～107％.证明β-兴奋剂类类生物芯片各项性能指标完全能满足乳与乳粉中β-兴奋剂类类残留半定量的快速筛查,为批量样品筛查提供了可靠的技术保证.

The antigen, antibody biochip for drug residues in milk and milk dairy is still rare in the detection of relevant reports, the main use of the experimental growth-promoting agent combination biochips, the choice of which β-stimulants, trenbolone, corticosteroids Simultaneous quantitative determination of its three residues. Investigated the biochip for the quantitative determination of growth promoter residues accuracy, precision, etc .in milk are able to meet the testing requirements. The linear range is 0 - 5 ng/mL; β-stimulants, trenbolone, corticosteroids LOD were 0.1,0.5,0.2 ug/kg. 2 horizontal spiked （2 times the detection limit of five times the detection limit with the added amount） recovery experiment; their recoveries between 99 to 130%; among the latter added recoveries than the former; through 2 times with 5 doubly labeled sample precision measurement results, the coefficient of variation of 14% or less. Through the determination of all the negative samples below the respective detection limits, quality control samples was measured to the reference value of 105 to 107%. Prove growth-promoting agent class biochip can meet the performance indicators of growth-promoting agentresidues in milk and milk dairy semi-quantitative rapid screening, screening for bulk samples to provide a reliable technical guarantee.