摘要
建立了乳制品中的安赛蜜、糖精钠、苯甲酸、山梨酸和脱氢乙酸同时检测的高效液相色谱分析方法.样品经沉淀剂沉淀蛋白,过滤后,使用C18反相色谱柱分离,以甲醇-乙酸铵水溶液为流动相,梯度洗脱,紫外检测器检测,检测波长230 nm下5种物质在30 min之内完全分离.在选定的条件下相关系数均大于0.9980,线形范围1～-100mg/kg,回收率为80.0%～107.7％.本方法用于乳制品中以上五种物质的测定,具有快速、简单、准确的优点.
A method for detecting of acesulfame-K, saccharin sodium, benzoic acid, sorbic acid and dehydroacetic acid in dairy products by HPLC is described. The sample was deproteinated and filtered, then separated on a Thermo C18 column with the flow phase （Methanol - ammonium acetate solution）, and detected by the absorbance at 230 nm. Five kinds of substances completely separated within 30 min. The correlation coefficients （r2） all above 0.9980 in the selected condition, the linear range is 1 - 100 mg/kg, and the recovery rate is 80.0% -107.7%. The method is rapidly, simply, and reliably.
出处
《中国乳品工业》
CAS
北大核心
2014年第2期58-59,64,共3页
China Dairy Industry
