毛细管电泳法定量分析贝母中几种生物碱

Quantitative analysis of several alkaloids in Fritillaria L. by capillary electrophoresis

目的 建立毛细管电泳法 (CE)测定贝母中浙贝乙素、西贝素、西贝苷等生物碱的含量测定方法。方法 以毛细管区带电泳模式 ,用内标法分析了 9种贝母药材中上述生物碱的含量。结果 3种生物碱分离完全 ,并有较好的回收率和重现性。结论 本法快速、简便、高效 。

Object To develop a method for the quantitative analysis of peiminine, imperialine and imperialine 3β D glucoside in Fritillaria L by capillary electrophoresis Methods 9 species of bulbus Fritillaria L. were analysed with internal standard by the mode of capillary zone electrophoresis Results The 3 alkaloids were separated completely with the method which showed good recovery and reproducibility Conclusion The method was proved to be quick, simple, and efficient, and provided a reliable basis for the quality control and evaluation of this plant medicine