卵胞浆内单精子注射和常规体外受精-胚胎移植的胚胎形态及临床结果比较

Comparison of embryo morphology and clinical outcome between embryos generated from conventional IVF and from ICSI

目的 :比较卵胞浆内单精子注射 (ICSI)和常规体外受精 -胚胎移植 (IVF-ET)两种助孕技术处理后的胚胎形态和临床结果。方法 :90对夫妇进行 93个 ICSI/ IVF治疗周期 (ICSI 45周期 ,IVF 48周期 ) ,比较两组周期平均获卵数 ,正常受精数 ,A、B级胚胎数和生化妊娠率 ,临床妊娠率。结果 :ICSI组周期平均获卵数及正常受精数 (分别为 1 1 .2 5 ,6 .6 0 )与 IVF组 (分别为 1 0 .6 7,6 .90 )相类似 (P>0 .0 5 ) ;周期 A、B级胚胎数 ICSI组3.31 ,IVF组 3.1 9,两组比较无显著差异 (P>0 .0 5 )。周期临床妊娠率 ICSI组 42 % ,IVF组 44 % ,两组比较无显著差异 (P>0 .0 5 )。周期“抱婴回家”率 ICSI组 36 % ,IVF组 31 % ,两组比较无显著差异 (P>0 .0 5 )。结论 :ICSI技术治疗严重男性因素引起的不育症可取得与常规

Objective: The aim of this study was to assess and compare the embryo morphology and clinical outcome between two methods where embryos were generated either from intracytoplasmic sperm injection (ICSI) or from conventional in vitro fertilization-embryo transfer (IVF-ET) Methods: Ninety-three consecutive cycles undergoing conventional IVF(45 cycls) or (48 cycls) therapy during June lst and December 31 th 1999 were studied. All eggs and embryos were cultured in Earle's or HTF medium supplemented with 10% inactived maternal serum in a humidified gas environment of 5% CO 2. In ICSI therapy oocytes were injected with sperm using standard ICSI protocols. In conventional IVF each oocyte was inseminated with approximately 50 000 motile sperms. The best quality embryos were transferred on day 2 (post-insemination), whereas supernumerary embryoswere frozen. Fertilization rate, embryo quality and clinical endpoints were assessed and statistical significance was tested via SPSS/PC 6.0. Results: The average and the mean infertile duration of female patients were comparable in the two treatment groups (IVF 30.9 yrs and 7.35 yrs, ICSI 30.1 yrs and 6.71 yrs).The number of oocytes collected or fertilized and the number of grade A or B embryos per cycle had no significant difference between the two methods. There was also no difference either in clinical pregnancy rate or in baby delivered rate per cycle. Conclusions: In this study most parameters were found to be similar. The ICSI and conventional IVF provided essentially similar reproductive outcomes.