bcl-2在鸡马立克氏病肿瘤中的表达

EXPRESSION OF bcl-2 IN MAREK’S DISEASE TUMOR

用来克亨SPF鸡复制马立克氏病 (Marek’sdisease ,MD)肿瘤模型 ,取肿瘤和相应正常组织 ,液氮保存。AGPC(AcidGuanidiniumThiocyanate Phenol Chloroform)法提取组织总RNA(TR NA) ,紫外测定其浓度 ,甲醛变性胶电泳观察其纯度。将一个含鸡bcl 2 1 2kb片段的质粒(PTTCB1) ,经转化扩增 ,提取质粒DNA ,限制性内切酶 (RE)消化 ,回收纯化bcl 2片段 ,放射性α 32P标记 ,制成探针。通过Northern分子杂交检测MD肿瘤组织中bcl 2的表达 ,并与相应的正常组织比较。结果 :鸡MD肿瘤组织和相应正常组织中均有bcl 2的表达 ;MD肿瘤组织中bcl 2的表达显著高于相应的正常组织。结合已进行的凋亡研究表明 ,bcl

Model of MD tumor was reproducted on SPF white Leghorn chicken,collected tumor and normal tissues,conserved in liquid nitrogen.TRNAs in the tissues were obtained by AGPC method,detected their concentration and analyzed by 1% denatured agrose gel containing formaldehyde.A plasmid(PTTCB 1) that contains chicken bcl 2 1 2 kb fragment was amplified,extracted and digested with restriction endonuclease(RE),the 1 2 kb fragment was recovered and purified with purigene kit,labeled probes with α 32 P.Expression of bcl 2 was analyzed with Northern blot hybridization.Results:(1)There were bcl 2 transcripts in MD tumor and relevent normal tissues;(2)Expression of bcl 2 in MD tumor was much higher than in normal tissues.Our findings indicates that expressive products of chicken bcl 2 promotes lyphomagenesis of MD by blooking apoptotic cell death.