解酒护肝方对不同体质大鼠酒精性肝损伤的保护作用

Study on the Protection of JieJiu HuGan Prescription against Alcoholic Liver Injury of Rats in Different Constitutions

目的:观察解酒护肝方对不同体质大鼠酒精性肝损伤的保护作用。方法:将大鼠随机分为正常组、阳虚模型组、阳虚预防给药组(温低组、温高组、寒低组、寒高组)、阴虚模型组、阴虚预防给药组(寒低组、寒高组、温低组、温高组)等11组,除正常组外,阳虚组采用30 mg/kg肌注氢化可的松,阴虚组采用甲状腺素水溶液25 mg/kg分别建立阳虚模型、阴虚模型,造模完成后针对体质预防给药,分高、低剂量组(按生药量计为4.15,0.83 g/kg),1小时后灌胃0.009 mL/g 56度红星二锅头,持续灌注5周后测定大鼠血清谷草转氨酶(AST)、谷丙转氨酶(ALT)水平。结果:与正常组比较,模型组与预防给药组的AST、ALT及AST/ALT比值均增高,提示有不同程度的肝损伤(P<0.05);违背体质用药的高剂量组血清AST、ALT增高明显(P<0.05);符合体质用药的高剂量组血清AST、ALT明显降低(P<0.05)。结论:针对体质的解酒护肝方能有效预防大鼠酒精性肝损伤。

Objective：To observe the protective effects of JieJiu HuGan prescription on alcoholic liver injury of rats with different constitutions. Methods： All the rats were randomized into 11 groups： normal group, Yang deficiency model group, medication groups of preventing Yang deficiency （low dose group of warm-nature prescription, high dose group of warm-nature prescription, low dose group of cold-nature prescription and high dose group of cold-nature prescription）, Yin deficiency model group, medication groups of preventing Yin deficiency （low dose group of warm-nature prescription, high dose group of warm-nature prescription, low dose group of cold-nature prescription and high dose group of cold-nature prescription）, except normal groups, the models of Yang deficiency were established by muscular injection of hydrocortisone （30 mg/kg） and the ones of Yin deficiency by intragastric administration of thyroxine aqueous solution （25 mg/kg）. According to the constitutions, the rat models were given with preventive medicine in different dosages （4.15, 0.83 g/kg, intragastric administration）, after one hour, the rats were perfused with Erguotou （112 degrees proof, 0.009 mL/g）, the levels of AST and ALT in the serum of the rats were detected after perfused for five consecutive weeks. Results：Compared with the normal group, AST, ALT and AST/ALT of the model group and the preventive medication groups were increased, which suggested liver injury in different degrees （P〈0.05）;AST and ALT were improved significantly in the high dosage group which were medicated against the constitution （P〈0.05）;AST and ALT were decreased remarkably in the high dosage group which were medicated based on the constitution （P〈0.05）. Conclusion： JieJiu HuGan prescription based on the constitution could effectively prevent alcoholic liver injury of the rats.