小鼠品系、给药剂量及次数对链脲霉素诱导1型糖尿病的影响

Effects of mouse strains,doses and times of Streptozotocin on inducing type 1 diabetes

目的考察小鼠品系、链脲霉素剂量以及给药次数对1型糖尿病模型成模率及稳定性的影响。方法将健康雄性ICR、C57BL/6J小鼠均随机分为正常对照组和链脲霉素200 mg/kg单次给药组,考察小鼠不同品系对链脲霉素诱导1型糖尿病的模型影响;将健康雄性C57BL/6J小鼠分随机为正常对照组、链脲霉素100mg/kg单次给药组及100 mg/kg多次给药组,考察链脲霉素不同给药次数对对链脲霉素诱导1型糖尿病的模型影响;将健康C57BL/6J雄性小鼠随机分为正常对照组和链脲霉素100、150、180、200 mg/kg单次给药组,考察链脲霉素不同剂量对链脲霉素诱导1型糖尿病的模型影响;末次注射链脲霉素3 d后检测各组小鼠空腹血糖值,以血糖值≥11.1 mmol/L为判定模型成立的标准。结果链脲霉素对ICR小鼠体质量和血糖值均没有影响;100 mg/kg多次给药诱导C57BL/6J小鼠1型糖尿病的成模率是80%;100、150、180、200 mg/kg单次给药诱导C57BL/6J小鼠1型糖尿病的成模率分别是50%、70%、80%和87.5%。结论 C57BL/6J小鼠在链脲霉素诱导小鼠1型糖尿病模型中较ICR小鼠更为敏感,多次腹腔注射小剂量（100 mg/kg）链脲霉素或单次腹腔注射大剂量（150~180mg/kg）链脲霉素均可造成稳定可靠的小鼠1型糖尿病模型。

[Objective] To study the effects of mouse strains, doses and times of Streptozotocin on rate and stabil- ity of type 1 diabetes model. [Methods] Healthy male ICR, C57BU6J mice were randomly divided into normal, Streptozotocin 200 mg/kg single dose group to study the effects of different strains of mouse on Streptozotocin-in- duced type 1 diabetes. Healthy male C57BL/6J mice were divided randomly into normal, Streptozotoein 100 mg/kg single, 100 mg/kg multiple group to compare the effects of different times of administration of Streptozotocin on Streptozotocin-indueed type 1 diabetes. Healthy C57BId6J male mice were randomly divided into normal, Streptozo- tocin 100, 150, 180, 200 mg/kg single dose group to investigate the effects of Streptozotocin on inducing type 1 dia- betes. Diabetes was confirmed by blood glucose that was ≥ 11.! mmol/L three days after injecting. [ Results ] Strep- tozotocin had no effect on body weight and blood glucose values of ICR mice. 100 mg/kg multiple injection induced 80% of C57 BL/6J mice with type 1 diabetes. 100, 150, 180, 200 mg/kg single injection induced 50, 70, 80, 87.5%of C57 BL/6J mice with type 1 diabetes respectively. [Conclusions] C57 BL/6J mice are more sensitive to Strepto- zotocin-induced type 1 diabetes than ICR mice. Multiple intraperitoneal injection of a small dose （100 mg/kg） Strep- tozotocin or single intraperitoneal injection of large doses （150-180 mg/kg） Streptozotocin can make a stable and re- liable mouse type 1 diabetes model.