摘要
目的探讨应用流式细胞术检测人类白细胞抗原B27(HLA-B27)在临床上对强直性脊柱炎(AS)的诊断价值。方法应用流式细胞术,选择来本院就诊的门诊、住院的AS患者(AS组)561例及健康体检者(健康对照组)1 000例,进行HLA-B27检测。结果两组间HLA-B27阳性率比较:AS组为88.95%,健康对照组为6.90%,两组比较差异有显著性(P〈0.01)。两组不同性别间HLA-B27检测阳性率比较:临床确诊的AS组561名患者中,HLA-B27表达阳性患者499名(88.95%),其中男性364名(64.88%),女性135名(24.07%),AS组男女比例约为2.7∶1,阳性率差异有显著性(P〈0.01);健康对照组1 000例中HLA-B27表达阳性患者69名(6.9%),其中男性39名(3.9%),女性30名(3.0%),健康对照组男女比例约为1.3∶1,阳性率差异无显著性(P〉0.05)。两组不同年龄段间HLA-B27检测阳性率比较:临床确诊的AS组561名患者,HLA-B27表达阳性患者中21~40年龄段298名(52.46%),21~40年龄段阳性率差异有显著性(P〈0.01),其他年龄段的阳性率无显著性差异(P〉0.05);健康体检组HLA-B27表达阳性患者各年龄段的阳性率无显著性差异(P〉0.05)。结论 HLA-B27与AS具有高度相关性,检测HLA-B27有助于早期发现AS患者,以实现早期诊断,早期治疗。
[ Objective ] To discuss the application flow cytometry(FCM) detection HLA B27(HLA-B27) in clinical of ankylosing spondylitis (AS) diagnostic value. [Methods] HLA-B27 detection was conducted by flow cytometry (FCM) in 561 patients of AS (AS group), and 1000 healthy individuals. [ Results ] Between the two groups HLA-B27 positive rate comparison: AS group is 88.95%, the healthy individuals group is 6.90%, two groups of comparisons differences are significant (P 〈0.01). In clinically diagnosed of 561patients with AS, HLA-B27expression positive patients with 499 (88.95%), of which 364 male patients (64.88%), the female 135 (24.07%), the positive ratio was statistical difference in males to females with HLA-B27 positive expression was about 2.7:1 (P 〈0.01). 1000 healthy individuals controls, HLA-B27expression positive patients with 69(6.9%), the male 39 (3.9%), the female 30 patients (3.0%), the positive ratio was no statistical difference in males to females with HLA-B27 positive expression was about 1.3:1 (P 〉0.05). Two groups between different age paragraph HLA-B27 testing positive rate comparison: In diniea[ty diagnosed of 561patients with AS, HLA-B27 expression positive patierits , 21-40 ages 298 (52.46%), the positive ratio was statistical difference in 21-40 age (P 〈0.01), and the other positive rate of age no statistieal significance (P 〉0.05). The healthy individuals group FILA-B27 expression positive patients of different age stages in the positive rate of no statistical significance (P 〉0.05). [ Conclusions ] The detection of HLA-B27 plays important roles in the early diagnosis and early treatment of patients with AS.
出处
《中国现代医学杂志》
CAS
CSCD
北大核心
2013年第36期56-59,共4页
China Journal of Modern Medicine