摘要
从浙江台州近海上层海水中分离到一株可产Macrolactin类大环内酯抗生素的海洋细菌,经形态特征分析及16S rRNA测序比对,鉴定并将其命名为解淀粉芽孢杆菌(Bacillus amyloliquefaciens)ESB-2。运用Minitab软件,通过Plackett-Burman和Box-Behnken实验及响应面分析对该菌生产Macrolactin B的发酵条件进行优化,得到该菌生产Macrolactin B的最佳发酵条件为:葡萄糖17.0mg/L,蛋白胨5mg/L,酵母粉1mg/L,FePO40.01mg/L,初始pH 6.0,培养温度27.2℃,接种量5%,装液量57.6%,培养时间4d,在优化发酵条件下,Macrolactin B的最高产量达到13.6?g/mL,为目前已报道的最高产量,为该活性物质药源问题的解决打下了良好基础。
A macrolactins-producing marine bacteria were isolated from seawater in Taizhou, Zhejiang Province. This strain was identified and named as Bacillus amyloliquefaciensESB-2 based on phenotypic characteristics and 16S rRNA gene sequencing. Minitab statistical software was applied to optimize the fermentation conditions for the production of macrolactin B fermented by ESB-2 strain in Plackett-Burman and Box-Behnken design. The optimal fermentation conditions for maximum macrolactin B production were Peptone 5mg/mL, yeast extract 1mg/mL, glucose 17.0mg/L, FePO4 0.01mg/mL, temperature 27.2℃, initial pH value 6.0, medium volume 57.6%, rotation speed 150r/min, inoculation 5% and fermented for 4 days. Under the optimization conditions, the concentration of macrolactin B was increased eight-folds and reached 13.6μg/mL.
出处
《海洋与湖沼》
CAS
CSCD
北大核心
2013年第6期1592-1596,共5页
Oceanologia Et Limnologia Sinica
基金
浙江省海洋生物技术产业科技创新团队资助项目,2012R10029-2号
