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海南省消除疟疾前期间日疟原虫环子孢子蛋白多态性研究及其分子进化关系分析

Study on the polymorphism in circumsporozoite protein of Plasmodium vivax before thecompletion on elimination program of malaria, in Hainan province
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摘要 目的分析海南省消除疟疾前期间日疟原虫环子孢子蛋白(CSP)基因型。方法采用PCR扩增特异性目的片段,基因测序,序列比对及进化树构建等方法。结果19份本地感染问13疟样本中18份为VK210,1份为VK247,VK210为优势亚型。与参考株相比,VK210又分为7个亚型,VK247仅有1个亚型。VK210各亚型中GDRAD(A)GQPA重复序列均存在不同数量的D或A点突变,属于高度突变区域;其余重复序列(GNGAGGQAA和GGNA)大多数仅发生基因数量的增减变化。海南省疟疾控制阶段和消除阶段间日疟VK210和VK247基因型构成比的差异无统计学意义(P〉0.05),进化树分析显示两基因型分别属于不同的进化簇。结论海南省消除疟疾前期仍存在两种基因型间日疟。与控制阶段相比,种型间构成比差异不显著,间日疟虫株消失结果相一致。 Objective To explore the polymorphism in circumsporozoite protein of Plasmodium vivax before malaria was eliminated in Hainan island. Methods PCR amplification, sequencing, and alignment methodologies were conducted and phylogenetic tree constructed. Results From all the cases, 19 of them belonged to two types,with 18 as VK219 type and 1 as VK247 type. VK210 type could be divided into seven kinds of subtypes but VK247 had only one type. Ratio of tropical strain with temperate stain in VK210 type was explored between the two stages: control or elimination. Phylogenetic tree was constructed by amino acid sequencings which clearly manifested that VK210 type and VK247 type belonged to different clusters. Conclusion Compared the proportion of two types in the control stage, there was no significant difference seen in the stage of elimination.
出处 《中华流行病学杂志》 CAS CSCD 北大核心 2014年第3期303-306,共4页 Chinese Journal of Epidemiology
基金 海南省自然科学基金(310174,813251)
关键词 间日疟原虫 环子孢子蛋白 基因多态性 Plasmodium vivax Circumsporozoite protein Polyraorphism
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  • 1Arnot DE, Bamwell JW, Tam JP, et al. Circumsporozoite protein of Plasmodium vivax: gene cloning and characterization of the immunodominant epitope [J]. Science, 1985, 230 (4727) : 815- 818.
  • 2Rosenberg R, Wirtz RA, Lanar DE, et al. Circurnsporozoite protein heterogeneity in the human malaria parasite Plasmodium vivax [J]. Science, 1989,245(4921 ) :973-976.
  • 3Goldman IF, Qari SH, Millet PG, et al. Circumsporozoite protein gene of Plasmodium simium, a Plasmodium vivax-like monkey malaria parasite [J]. Mol Biochem Parasitol, 1993,57 (1) : 177- 180.
  • 4Cochrane AH, Nardin EH, de Arruda M, et al. Widespread reactivity of human sera with a variant repeat of the circumsporozoite protein of Plasmodium vivax [J]. Am J Trop Med Hyg, 1990,43(5) :446-451.
  • 5Gopinath R, Wongsrichanalai C, Cordon-Rosales C, et al. Failure to detect a Plasmodium vivaxlike malaria parasite in globally collected blood samples [J]. J Infect Dis, 1994, 170 (6) : 1630- 1633.
  • 6黄天谊,黄亚铭,王小力,黎学铭,张再兴,王善青,王光泽,潘波,黄志彪,付伟忠,刘惠,高琪,张绍清,尚乐园,许国君,沈毓祖,刘涛,李蓬,曾风秀,车莹.我国间日疟原虫基因型种群结构及其地理分布[J].中国寄生虫学与寄生虫病杂志,2001,19(5):260-264. 被引量:23
  • 7王光泽,华德,王善青,林翠芬,林世干,蓝秀汉,李才旭,黄天谊,司有忠.海南省间日疟原虫环子孢子蛋白基因分型及地理分布[J].中国寄生虫病防治杂志,2001,14(3):174-175. 被引量:4
  • 8Yien-Kyoung C, Kyung-Mi C, Mi-Hyun E Rapid dissemination of newly introduced Plasmodium vivax genotypes in South Korea [J]. Am J Trop Med Hyg, 2010,82 (3): 426-432.
  • 9周水森,王漪,李雨.2010年全国疟疾疫情分析[J].中国寄生虫学与寄生虫病杂志,2011,29(6):401-403. 被引量:133
  • 10王光泽,王善青,胡锡敏,蒙锋,李雨春,曾文,蔡红林.2010年海南省疟疾疫情分析[J].中国血吸虫病防治杂志,2012,24(3):369-370. 被引量:10

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