小鼠心脏移植技术的改良

Improved technique of cervical heart transplantation model in mice

目的 探讨小鼠颈部心脏移植模型手术技术的改进。方法 采用供心主动脉与受体颈总动脉侧壁长度相近的切口行端－侧连续吻合的方法进行４０次小鼠颈部心脏移植。结果 应用该方法使供心总缺血时间缩短至３０ｍｉｎ；７ｄ存活率提高到９７％。结论 新技术的采用降低了小鼠颈部心脏移植模型制作的难度，是一种简单实用的新方法。

Objective To develop a model of mouse cervical heart transplantation by using improved techniques. Methods The aorta of the donor heart and the right common carotid artery of the recipient were anastomosed using the end - to - side suture technique. Results The total ischeamic time of the graft was shortened to 30 min, and the 7 - day sur-vival rate of the recipient increased to 97 % by using this technique. Conclusion Cervical heart transplantation model in mice is a new, simple and useful technique.