凝胶色谱-气相色谱-串联质谱法筛查茶叶中27种禁用农药

Analysis of 27 banned pesticides in tea by GPC and gas chromatography-triple quadrupole mass spectrometry

建立了茶叶中27种国家禁用农药的气相色谱-串联质谱分析方法。以27种国家禁用农药为目标分析物，样品经环己烷-乙酸乙酯超声提取、凝胶渗透色谱净化后进行定性和定量分析。方法检出限为O．02～2．82μg/kg，方法定量限为0．07～9．40μg／kg，在0．01～0．20mg／L范围内方法线性相关系数均大于0．991，75％以上的农药的回收率在70％-110％之间，相对标准偏差在0．53％～7．1％之间。方法适用于茶叶中多种农药残留的检测分析。

A multi-residue method was developed for rapid determination of 26 pesticides in tea by GC-MS/MS in multiple reaction monitoring mode. Target analytes were extracted by ultrasonic with cyclohexane-ethyl acetate, purified by gel permeation chromatography and detected by GC-MS/MS. Good linearity was obtained in the range of 0. 01 -0. 20 mg/L. The limits of deteetion（LOD） were between 0. 02μg/kg and 2. 82μg/kg and limits of quantification（LOQ） were between 0. 07μg/kg and 9. 40μg/kg. The correlation coefficients were greater than 0. 991. The mean recoveries in tea through the method ranged from 70% to 110%, and the relative standard deviations（RSDs） of 0. 53% -7. 1%. The method can be applied to the detection of pesticide residues in tea.