肝移植后人血清中差异表达蛋白质的纳升超高效液相色谱-电喷雾串联质谱鉴定

Identification of the differentially expressed proteins in human serum after liver transplantation by nanoUPLC-ESI-MS /MS

建立了基于质谱的人肝移植后血清中差异表达蛋白质的分析方法，肝移植前后的人血清蛋白质用双向凝胶电泳分离，AgNO，染色，差异蛋白质斑点用胰蛋白酶进行胶内酶解后，采用纳升超高效液相色谱-电喷雾串联质谱进行鉴定。鉴定了8个显著差异表达的蛋白质，Mascot检索分值101～1543分，肽段匹配数5～34个，序列覆盖率10％～43％。所发现的差异蛋白质多与免疫调节有关，可为深入研究肝移植术后免疫排斥的分子机理提供线索。

In this report the mass spectrometry-based method was established for analysis of differentially expressed protein in human serum after liver transplantation. The proteins in serum before and after liver transplantation were separated by two-dimensional gel electrophoresis with silver staining, and the differevtial proteins on the gel were done in gel digestion with trypsin and then identified by nanoUPLC-ESI-MS/MS. In this study 8 differentially expressed protein spots were peptide matching number was 5 - 34 and sequen expressed proteins are related to immune regulatio study of liver allograft rejection mechanism. identified clearly. The Mascot ce coverage was 10% - 43%. n mostly, and therefore it may search score was 101 -1543, It is found that differentially provide clues for the in-depth