芥子中甲代烯丙基异硫氰酸酯对k562细胞的抑制与诱导

Inhibition and Inducing of k562 Cells by Methallyl Isothiocyanate Extracted from Mustard Seeds

从海南十字花科植物芥菜种子中提取异硫氰酸酯类化学成分甲代烯丙基异硫氰酸酯(MITC),并将其作用于k562细胞,用MTT比色法测定增殖抑制效果,应用瑞式姬姆萨、4',6-二脒基-2-苯基吲哚(DAPI)、罗丹明123染色观察细胞形态。结果表明,MITC作用k562细胞呈现一定的量效关系,MITC体积分数为120μmol·L-1,作用时间为96 h时,抑制率达到最高,为(87.5±1.4)%。MITC作用k562细胞24,48,72,96,120 h的半数抑制体积分数(IC50)分别为250.007,100.584,39.030,54.701,88.719μmol·L-1。MITC处理的K562细胞出现凋亡形态特征:细胞皱缩,细胞核固缩,细胞核向内凹陷,线粒体电势下降。根据细胞形态变化初步判断MITC诱导k562细胞凋亡。

Methallyl isothiocyanate （MITC） were extracted from the seeds of mustard （Brassica juncea）, a cru- ciferous plant, which grows in Hainan Province, China. The inhibition effect of MITC on proliferation of k562 cells was determined by a MTT assay, and the cell morphology was observed by using Wright-giemsa, DAPI and Rodanmin 123 staining. MITC showed a concentration-response relationship with k562 ceils in cell proliferation in vitro. The k562 cells were inhibited at the highest rate （87.5 ±1.4） % when treated with the MITC at 120 μmol·L^-1 for 96 h. The k562 cells treated with MITC for 24, 48, 72, 96, 120 h gave respective IC50 of 250.007, 100.584, 39. 030, 54. 701, 88. 719μmol·L^-1. And the cells shrank with nuclear chromatin conden- sation, nuclear inward depression and low mitochondrial potential. These morphological changes suggest that k562 cell apoptosis were induced by MITC.