测定无乳链球菌灭活疫苗浓度的分光光度法

Establishment of Spectrophotometric Method for Concentration Determining of the Inactivated Vaccine Against Streptococcus agalactiae

以无乳链球菌灭活疫苗为研究对象,利用分光光度计建立了快速准确测定该灭活疫苗浓度的方法.研究结果表明,波长为600 nm处无菌生理盐水及稀释后的无乳链球菌灭活疫苗均无特征吸收峰,吸光度变化平缓,此时无乳链球菌灭活疫苗的吸光值（X）与其浓度（Y）呈正相关（r =0.998 3,df=6,P＜0.01）,其关系曲线为：y=11.277x-2.022 9.无乳链球菌菌液经φ=0.4％甲醛灭活3h的A600值基本稳定;选择4名实验员对该方法进行重复检测,结果显示所测疫苗A600值的RSD为0.5％ ～1.2％,且符合上述相关性方程;说明利用分光光度计法测定经甲醛灭活的无乳链球菌疫苗浓度,其结果较准确、重复性较好,具有简单快速的特点.

A method for rapid determination of the concentration of inactivated vaccine against the bacteria, Streptococcus agalactiae was established by spectrophotometry. At the wavelength of 600 nm both physiological saline and inactivated vaccine showed smooth absorbance change and no characteristic absorption peak wave- length. The A6oo value （X） was positively correlated with the concentration of the vaccine （Y） （ r = 0.998 3, df= 6, P 〈 0.01 ）, as revealed by the equation y = 11. 277x - 2. 022 9 ; the Ar0o value of vaccine remained sta- ble when it was inactivated by 0.4% formaldehyde for 3 h. The determination was repeated by 4 laboratory tech- nicians, and the results showed the A600 value was in accordance with the above correlation equation and its RSD ranged between 0. 5%--1. 2% . This indicates that this method for determination of the concentration of inactiva- ted vaccine against the bacteria was very stable, repeatable, easy to handle and rapid.