白花树花粉生活力检测方法与贮藏特性研究

Detection Methods of Pollen Viability and Storage Characteristics of Styrax tonkinensis

以江西吉水种源的白花树花粉为研究材料,进行了扫描电子显微镜下的花粉形态结构观察以及采用离体培养基法、I2-KI和TTC染色等花粉生活力测定方法的比较研究,探讨了不同贮藏条件对花粉萌发的影响。研究结果表明:白花树花粉粒为长球型,具3孔沟,外壁面具细网状纹饰;花粉平均极轴长52.33±0.577μm,平均赤道轴长26.89±0.502μm;3种花粉生活力测定方法中以离体培养基法最有效,花粉萌发的最佳组合为:10%蔗糖+0.05 g·L-1硼酸+100 mg·L-1GA3+3 h;花粉最佳贮藏条件为-80℃密封保存,贮藏180 d后花粉的萌发率仍可达40.97%。

With the Styrax tonkinensis from Jishui, Jiangxi Province, the morphological structure of pollen was ob- served under SEM. The storage methods determining the pollen viability were compared among culture in vitro, 12 - KI staining and TTC staining. Meanwhile, the effects of different storage condition on pollen germination was inves- tigated. The results show that the pollen of S. tonkinensis with microreticulate ornamentation in outer wall is 3-col- pate and prolate. The average polar and equatorial axis of the pollen is 52.33 ±0.577 μm and 26.89 ±0. 502 μm. The method of culture in vitro is the most effective to the determination of pollen viability. The best combination for pollen germination is 10% sucrose, 0.05 g· L ^-1 boric acid, 100 mg·L ^-1 GA3, 3 h. -80℃ sealed storage is the best storage condition to keep the vitality of pollen. After 180 days＇ storage, the germination rate of the pollen is still as high as 40.97%.