大孔吸附树脂对白蜡虫多糖脱色及抗氧化活性的影响

Decolorization and Antioxidant Assay of Polysaccharide from White Wax Scale Using Macroporous Resin

试验研究了大孔吸附树脂对白蜡虫粗多糖脱色效果的影响和脱色前后多糖抗氧化活性的变化。通过静态吸附法确定大孔吸附树脂NKA-9较适合白蜡虫粗多糖脱色,动态吸附法确定白蜡虫多糖脱色条件为30℃下10 mg·mL-1多糖溶液(pH值8.0)以1 BV·h-1流速上样1 BV后,以5 BV水洗脱,脱色率为71.4%、脱蛋白率51.9%、多糖收率为60%。体外细胞抗氧化结果表明:脱色前多糖溶液仅在1 000μg·mL-1浓度时能提升过氧化氢损伤人神经母细胞瘤细胞的存活率,而脱色后多糖溶液在10 1 000μg·mL-1范围内均能提升模型细胞存活率,其中,1 000μg·mL-1的脱色白蜡虫多糖与模型组的差异显著(P<0.01),结果表明,脱色后多糖抗氧化能力增加。

The decoloring effect of 12 macroporous resins on crude polysaccharide extracted from white wax scale, Ericeruspela pela （ Chavannes）, was studied. The cellular antioxidant activity of crude and decolorant polysaccha- ride on proliferation effect of SH-SY5Y cells induced by hydrogen peroxide were evaluated. The resin NKA-9 was selected for kinetic study based on the results of adsorption properties comparing of macroporous adsorption resins and the static adsorption test. The dynamic adsorption and desorption experiments were conducted to establish the optimum parameters of NKA-9 as ： one volume solution of crude polysaccharide was loaded in column at the flow rate 1 bed volume per hour under the condition of 30 ℃ and pH 8.0. The decolorant polysaccharide was collected by washing adsorbate-laden column using five bed volume of deionized water. The decoloration rate was 71.4%, the yield of polysaccharide was 60% , the deproteinization rate was 51.9%. The results of cellular antioxidant activity assay showed that the cell livability could be increased only at the concentration of 1 000 μg · mL^-1 crude polysac- charide. But the decolorant polysaccharide at the concentrations of 10 - 1 000 μg · mL^-1 could reduce the cell death rate of SH-SYSY. Among them, the decolorant polysaccharide at the concentrations of 1 000μg · mL^-1 could reduce the cell death rate notably. The cellular antioxidant activity of decolorant polysaccharide was enhanced after decoloring by NKA-9.