摘要
采用固相萃取(solid phase extraction, SPE)柱对样品中维生素B12进行浓缩与净化,提出了一种可灵敏测定营养强化食品中维生素B12含量的超高效液相色谱-串联质谱(ultra high performance liquid chromatography-tandem mass spectrometry, UPLC-MS/MS)方法,并通过实验考察了不同固相萃取柱对维生素B12含量测定结果的影响.实验结果表明,采用HLB(hydrophile-lipophile balance number)固相萃取柱进行富集与净化,维生素B12在0.5~100.0μg/L浓度范围内与其响应值呈良好的线性关系,检出限为1.0μg/kg,说明该方法具有分析速度快、灵敏度高、重复性好的优点,适用于对营养强化食品中维生素B12含量的测定.
A simple, fast and accurate method for determination of vitamin B12 in fortified foods is developed using ultra high performance liquid chromatography-tandem mass spec-trometry (UPLC-MS/MS). The sample is concentrated by solid phase extraction (SPE) column. The linear range of the method is from 0.5 to 100.0 μg/L with a correlation co-efficient of 0.999 9. The detection limit is 1.0 μg/kg. The proposed method exhibits high sensitivity and good reproducibility for determining vitamin B12.
出处
《上海大学学报(自然科学版)》
CAS
CSCD
北大核心
2014年第1期114-119,共6页
Journal of Shanghai University:Natural Science Edition
基金
国家自然科学基金资助项目(61171033)