纯阳子对兔髂动脉球囊成形术后血管局部c-myc基因表达的影响

Effects of pyraceantha roem on c-myc gene expression after ballon angioplasty in the rabbits iliac arteries

目的 :探讨具有抗氧化作用的纯阳子对兔髂动脉球囊成形术后血管局部 c- myc基因表达的影响及c- myc表达的高峰期与血管壁解剖结构之间的关系。方法 :32只日本大耳白兔分成 4组 (每组 8只 ) :A组 :左侧髂动脉为假手术组 ,右侧髂动脉为正常对照组 ;B组 :双侧髂动脉球囊成形术组 ;C组 :球囊成形术加维生素 C和 E组 ;D组 :球囊成形术加纯阳子治疗组 ,均于术前用药 7d。应用原位杂交的方法 ,观察纯阳子对球囊损伤后 2 h血管局部 c- m yc基因表达的影响及其与血管壁解剖结构的关系。结果 :A组左、右髂动脉均未见 c- m yc基因的表达。B组近管腔部可见一层阳性显色 ,中膜沿平滑肌细胞排列方向可见阳性显色。C组及 D组均见近血管腔部有一层阳性显色 ;中膜沿平滑肌细胞排列方向也见一些散在的表达 ,两组之间无明显差异 ;但与 B组相比 ,颜色明显变浅。结论 :纯阳子能抑制兔髂动脉球囊损伤后 c- m yc基因的过度表达 ;球囊损伤后 2 h(损伤后 c- myc基因表达的高峰期 ) ,c- myc基因在靠近管腔处表达最显著。

Objective: To investigate the effects of pyraceantha roem on c myc gene expression and the relationship between c myc gene expression and vessel architecture after balloon angioplasty in the rabbitsiliac arteries.Method:Thirty two rabbits were divided into four groups:①group A (n=8):rabbit right iliac artery was as control and the left one got sham operation;②group B (n=8):balloon angioplasty was performed in biiliac arteries(BAB);③group C (n=8):balloon angioplasty (BAB) plus vitamin C+E;④group D (n=8):BAB plus pyraceantha roem.Vitamins and pyraceantha roem were administerad 7 days before balloon injury and continued until the rabbits were killed 28 days after injury.Expression of c myc gene were examined at 2 h after balloon angioplasty of iliac arteries in rabbits by in situ hybridization.Result:①group A:there was no expression of c myc gene in the iliac arteries.②Compared with the group C and group D,group B had the most significant c myc gene expression on the media near the lumen of vessel. ③There was no significant difference between group C and group D.Conclusion:Pyraceantha roem can inhibit expression of c myc gene after balloon injury,and expression of c myc gene at the peak effect was the most significant on the media near vessel lumen.