鄂尔多斯地区蒙古族人群耐甲氧西林金黄色葡萄球菌基因分型

Genotype of methicillin-resistant Staphylococcus aureus from Mongolian in Ordos

目的：探讨鄂尔多斯地区蒙古族人群耐甲氧西林金黄色葡萄球菌（MRSA）的分子流行病学特点，明确本地区MRSA基因型别及其分布规律。方法收集2009年1月至2011年8月临床分离的MRSA菌株54株。应用多重聚合酶链反应（PCR）对MRSA菌株进行葡萄球菌染色体mec（SCCmec）基因分型、杀白细胞毒素（PVL）毒力基因（pvl）和多位点序列分型（MLST）检测。结果 MRSA菌株的SCCmec Ⅰ～Ⅴ基因分型分别占0．00％、50．00％、46．30％、1．85％和1．85％；检测出1株pvl基因阳性；24株MRSA菌株MLST分型显示，ST23913株（54．17％），ST59株（37．50％），ST59和ST7各1株（4．17％）。结论鄂尔多斯地区蒙古族人群MRSA菌株以SCCmec Ⅱ型和SCCmecⅢ型为主。

Objective To investigate the molecular prevalence characteristics of methicillin-resistant Staphylococcus aureus （MRSA）from Mongolian in Ordos,and to investigate the genotypes and distribution pattern of MRSA isolates. Methods A total of 54 MRSA isolates were collected from January 2009 to August 201 1 .The multiplex polymerase chain reaction（PCR）was used to determine Staphylococcal cassette chromosome mec （SCCmec）genotypes,Panton-Valentine leukocidin （PVL）gene （pvl）and multi-locus sequence typing （MLST）of MRSA isolates.Results The main SCCmec Ⅰ-Ⅴ genotypes of MRSA isolates,accounted for 0.00%,50.00%,46.30%,1 .85% and 1 .85%, respectively.The pvl gene had been discovered in only 1 isolate.Among 24 MRSA isolates,MLST results showed that 13 isolates were ST239（54.17%）,9 isolates were ST5（37.50%）,1 isolate was ST59 （4.17%）,and 1 isolate was ST7 （4.1 7%）.Conclusions The main genotypes of MRSA are SCCmecⅡand SCCmecⅢfrom Mongolian in Ordos.