细胞松弛素B对瘢痕增生的影响

Effects of cytochalasin B scar hypertrophic

目的 研究微丝功能对正常皮肤及增生性疤痕成纤维细胞胶原酶及金属蛋白酶组织抑制因子 1 mRNA表达水平的影响。方法 采用细胞培养， Northern blot分析等方法，以α 32P dCPT标记的胶原酶及 TIMP 1 cDNA为探针，检测正常皮肤及增生性瘢痕成纤维细胞胶原酶及金属蛋白酶组织抑制因子 1mRNA表达水平的变化。结果 用细胞松弛素 B破坏微丝后，正常皮肤及增生性瘢痕成纤维性细胞的胶原酶及 TIMP 1 mRNA含量明显升高。 结论 微丝骨架可能在基因转录水平参与对成纤维细胞合成细胞外基质的调控。

Objective To investigate the roles of microfilaments in the expression of collagenase and TIMP 1 in normal and hypertrophic scar.Method Cell culture and Northern blothybridization methods were used in this study.Result After the disruption of microfilanents with cytochalasin B,mRAN contents of collagenase and TIMP 1 both increased significantly in normal And hypertrophic scar fibroblasts.Conclusion The microfilament cytoskeleton may regulate the synthesis and degradation of ECM,which may be a mechanism of gene regulation during wound healing.