玉米黄色素对人卵巢透明癌细胞ES-2侵袭、迁移、凋亡及周期的影响

Effect of Maize Yellow Pigment on Invasion, Migration, Proliferation and Cell Cycle of Ovarian Carcinoma Cell Line ES-2

从玉米蛋白粉中提取、纯化得到玉米黄色素,研究其对人卵巢透明癌细胞ES-2的侵袭、迁移、凋亡及周期的影响。MTT法检测玉米黄色素作用于MDA-MB-231、PC-3、ES-2和A549细胞后的细胞活性;分别通过transwell体外侵袭试验、transwell体外迁移试验、流式细胞术检测玉米黄色素干扰对人卵巢透明癌细胞ES-2的侵袭能力、迁移能力、细胞凋亡和细胞周期的影响。结果显示,玉米黄色素对上述几种肿瘤细胞的生长均有抑制作用,其中对ES-2影响最为显著。当给药浓度达到100μg/mL时,对细胞的侵袭和迁移抑制率分别为52.59%和55.76%;流式细胞术检测结果表明,玉米黄色素浓度增加至100μg/mL时,G1期、S期和G2期细胞分别为85.33%、11.64%、3.21%,更多的ES-2细胞被阻断在G1期,且细胞凋亡率增加到13.85%。综上所述,玉米黄色素能够诱导ES-2细胞发生凋亡,阻断细胞周期,并抑制细胞的侵袭和迁移。

Maize yellow pigment was extracted from corn protein powder, and the possible effects on the proliferation of MDA-MB-231, PC-3, ES-2 and A549 cell lines were investigated by MTT assays, the effects on cell invasion and migration were studied by transwell assay, and its influences on cell cycle and cell apoptosis were observed by flow cytometry. Results showed that maize yellow pigment specifically inhibited the viability of MDA-MB-231, PC-3, ES-2 and A549 cell lines, especially on ES-2. The invasion and migration abilities were decreased to 52.59% and 55.76%, respectively, when the concentration of maize yellow pigment reached 100 μg/mL. The cells of G1 phase, S phase and G2 phase decreased to 85.33%, 11.64% and 3.21%, respectively, with the increase of maize yellow pigment concentration. The cell cycle of more ES-2 were arrested at Glphase. In summary, maize yellow pigment can induce apoptosis, arrest cell cycle and inhibit cell proliferation and migration of ES-2 cells.