摘要
研究诺丽果提取物抗氧化活性及其心肌细胞的保护作用,验证其对于心力衰竭的治疗效果。实验通过ABTS,DPPH,羟自由基清除率、铜离子还原力、总还原力等五种抗氧化实验测定诺丽果提取物及从提取物中分离所得两种单体化合物的抗氧化能力。通过流式细胞仪,倒置荧光显微镜检测诺丽果提取物心肌细胞保护作用,并应用高效液相色谱检测受损心肌细胞能量生成情况。抗氧化实验结果:维生素C>正丁醇萃取物>芦丁>黄酮醇。同时在终浓度均为100 mg/L条件下,诺丽果组心肌细胞凋亡率(19.34%±2.24%)和维生素C组(21.51%±3.24%)效果相当(P>0.01),而且诺丽果提取物可促进受损心肌细胞能量生成,其心肌细胞ATP含量37.26±4.33μg/g(24 h),38.247±4.25μg/g(48 h)显著优于维生素c组20.14±3.28μg/g(24 h),22.441±3.32μg/g(48 h)(P<0.01)。因此诺丽果提取物是安全的天然抗氧化剂,具有抗氧化活性和保护心肌细胞作用,可调节受损心肌细胞能量代谢,延缓心力衰竭进程。
The protective effect of Noni extract on myocardial cell were investigated, and verified by the treatment of heart failure. The antioxidative activities of the noni extracts and two compounds separated from the extracts were studied and detected by five methods, namely elimination of ABTS, DPPH and hydroxyl free radical scavenging activities, cupric reducing power and total reducing power. The protective effect of myocardial cell was detected by flow cytometry and inverted fluorescence microscope, meanwhile ATP generaion fi＇om damaged myocardial cell was detected by HPLC. The order of the antioxidantive activities of the four compounds were shown as vitmin C 〉n-BuOH 〉 rutin 〉 flavonol glycoside. No difference of the myocardial apoptosis rate hwas found between the noni extracts（19.34%±2.24%） and vitamin C （21.51±4：3.24%） at the fmal concentration of 100 mg/L （P〉0.01）. The noni extracts promoted the damaged myocardial cells. And myocardial ATP content 37.26±4.33 μg/g （24 h）, 38.247±4.25 μg/g （48 h） was significantly higher than vitmin C 20.14±3.28 μg/g （24 h）, 22.441±3.32 μg/g （48 h） （P〈0.01）. Thus noni extracts was a kind of safety antioxidant. The extracts could adjust the energy synthesis of the damaged myocardial cells and delay the process of heart failure.
出处
《现代食品科技》
EI
CAS
北大核心
2014年第2期31-36,共6页
Modern Food Science and Technology
基金
教育部高等学校博士学科专项科研基金(20102327110002)
黑龙江省中医药科研项目(ZHY12-Z111)
