胆源性胰腺炎大鼠胰腺胆囊收缩素受体变化的实验研究

The alteration of cholecystokinin receptors in the pancreas in rats with experimental biliary pancreatitis

目的 探讨胆源性胰腺炎 (biliarypancreatitis,BP)大鼠胰腺胆囊收缩素受体 (cholecys tokininreceptor,CCK R)和血浆胆囊收缩素八肽 (cholecystokininoctapeptide ,CCK OP)的变化及其之间的关系。方法 采用放射受体分析法检测 36只BP大鼠及 36只对照组大鼠胰腺组织膜CCK R和竞争性酶联免疫法检测其血浆CCK OP的含量。结果 胆胰管结扎后 3h、6h及 2 4h胰腺组织膜CCK R的最大结合容量 (Bmax)值为 179 0± 1 1、119 0± 1 8、及 114 0± 1 4fmol/ml;平衡解离常数Kd值为0 2 6± 0 0 1、0 13± 0 0 7、0 14± 0 0 1nM ;6h及 2 4hBmax值和Kd值与对照大鼠存在显著差异 (P均 <0 0 5 )。BP大鼠血浆CCK OP含量各为 12 5 5± 2 10、0 81± 0 16、0 79± 0 2 4PM/L ;与正常大鼠相比 ,胰腺CCK R在术后 6h及 2 4h期间结合位点数减少 ,亲合力增大 (P <0 0 5 ) ;血浆CCK OP含量仅在术后 3h与对照组大鼠差异有显著性意义 (P <0 0 5 )。结论 BP大鼠发病过程中 ,胰腺CCK R及血浆CCK

Objective To evaluate the alteration of cholecystokinin receptor in the pancreas and plasma cholecystokinin octapeptide in rats with biliary pancreatitis (BP), and the relation between cholecystokinin octapeptide and cholecystokinin receptor. Method By means of radioligand binding assay and competitive enzyme immunoassay, the pancreatic cholecystokinin receptor and plasma cholecystokinin octapeptide were investigated in rats with biliary pancreatitis reproduced by pancreatic bile duct ligation.Results At 3 hr , 6 hr , 24 hr after the ligation, the maximal binding capacity (Bmax) of CCK R was 179 0±1 1, 119 0±1 8, and 114 0±1 4 fmol/ml in BP rats respectively. The dissociation constant(Kd) was 0 26±0 01, 0 13±0 07, and 0 14±0 01 nM in BP rats respectively. Compared with normal rats the Bmax and Kd in BP rats were significantly different at 6 h and 24 h (all P <0 05). The contents of cholecystokinin octapeptide in plasma were 12 55±2 10, 0 81±0 16, 0 79±0 24 PM/L in BP rats respectively, which was statistically different from control at 3 h ( P <0 05) .Conclusions Significant decrease of Bmax and Kd was observed in BP rats. The content of pancreas cholecystokinin octapeptide in plasma increased in BP rats at 3hr( P <0 05). These results suggest that alteration of pancreatic cholecystokinin receptor and plasma cholecystokinin octapeptide may play an important role in the pathogenesis of biliary pancreatitis.