Pim-1在人宫颈鳞癌组织中的表达及临床意义

Expression and Clinical Significance of Pim-1 in Human Cervical Squamous Cell Carcinoma Tissues

目的:研究Pim-1基因及其蛋白在宫颈鳞癌组织中的表达水平,探讨其与宫颈鳞癌临床特性之间的关系。方法:采用逆转录-聚合酶链反应(RT-PCR)半定量方法和免疫组化学法,检测46例宫颈鳞癌组织和10例正常宫颈组织标本中Pim-1基因及蛋白的表达情况。结果:宫颈鳞癌组织中Pim-1基因与蛋白的表达水平明显高于宫颈正常组织(P<0.05);Pim-1蛋白在宫颈鳞癌组织中的表达与组织学分级、临床分期和淋巴结转移密切相关(P<0.05)。结论:原癌基因Pim-1及其蛋白在宫颈鳞癌组织中过度表达,可能与宫颈鳞癌的发生、发展有关。

Objective：To study the gene and protein expressions of Pim-1 in human cervical squamous cell carcinoma tissues,and to analyze the relationship between expressions and clinical characteristics. Method：Semi-quantitative reverse transcription polymerase chain reaction（RT-PCR） and immunohistochemistry analysis were used to detect the gene and protein expressions of Pim-1 in 46 cervical squamous cell carcinoma tissues and 10 normal cervical tissues;and their relations with clinical characteristics were analyzed. Result：The gene and protein expressions of Pim-1 in cervical squamous cell carcinoma tissues were significantly higher than that in the normal cervical tissues（P＆lt;0.05）;The protein expressions of Pim-1 in human cervical squamous cell carcinoma tissues were significantly related with pathologic grade,FIGO stage and lymph node metastasis of cervical squamous cell carcinoma（P＆lt;0.05）. Conclusion：Pro-oncogene Pim-1 and their protein are aberrant expressed in human cervical squamous cell carcinoma tissues, and they may be important for the occurrence and development of cervical squamous cell carcinoma.