四川东北地区女性人乳头瘤病毒感染的型别分布

Human papillomavirus genotypes in women of Northeast Sichuan

目的调查引起川东北地区女性泌尿生殖道感染的人乳头瘤病毒(HPV)的基因型别分布状况,为该地区HPV感染的诊断和防治提供依据。方法采用聚合酶链反应和基于反向膜杂交的基因芯片技术对临床送检的1 193例女性泌尿生殖道感染标本进行HPV检测。结果 1 193例标本中有586例为HPV阳性,HPV总感染率为49.12%;在被检测的23种HPV DNA基因亚型中有21种被检出。在586例HPV阳性感染者中,单一亚型HPV感染率为61.43%,两型混合HPV感染率为25.09%,多型(3型及3型以上)混合HPV感染率为13.48%;HPV亚型分布情况为:HPV6型30.55%、HPV11型27.99%、HPV16型28.84%、HPV58型12.97%、HPV43型11.09%、HPV33型8.02%、HPV18型5.97%、HPV52型5.29%,其他HPV亚型的感染率均低于5.00%;对不同年龄段女性而言,HPV总感染率、高危型HPV感染率及低危型HPV感染率的组间比较差异均有统计学意义(P<0.05)。结论川东北地区女性HPV感染率高,且以HPV单一亚型感染为主;HPV16为高危型HPV感染的主要型别,其次为HPV58和HPV33;HPV6和HPV11为低危型HPV感染的主要型别;HPV各亚型的感染率与年龄有一定关系。

Objective To investigate the distribution of human papillomavirus （HPV） genotype in women with genitourinary tract infection（GTI） in Northeast of Sichuan province and provide evidence for diagnosis and prevention. Methods Gene chip lech- nology was performed to examine HPV-DNA of each collected sample in 1 193 women with GTI. Results 586 samples had positive HPV among 1 193 samples,with a positive rate of 49.12%;21 HPV genotypes were detected successfully in 23 types of HPV DNA subtypes tested. Among 586 samples of HPV infection, 61.43 %were infected by single HPV genetype, 25.09 % were infected by two HPV genetypes and 13.48% were infected by multiple HPV genetypes;In the rate of HPV genetype infection, the percentages of HPV6,HPVll,HPV16,HPV58, HPV43,HPV33, HPV18, HPV52 were 30. 55%,27. 99%,28. 84%,12.97%,11. 09%, 8.02 %, 5. 97 %, 5.29 %respectively, and the rates of other HPV genetypes infection were less than 5.00 % ;There were significant differences in the infection rates of HPV and high risk HPV subtypes and low risk HPV subtypes among different age groups of women. Conclusion HPV infection was substantially severe and the major HPV infection types were multiple HPV subtypes infee tions in northeast of sichuan women;the most frequent infection of high risk HPV subtypes was HPV16, followed by HPV58 and HPV33. HPV6 and HPVll subtypes were the major HPV subtypes among the low risk HPV infections.