粉防己碱、甲基莲心碱和蝙蝠葛碱增强长春新碱诱导人乳腺癌MCF-7多药耐药细胞凋亡

POTEINTATION OF VINCRISTINE INDUCED APOPTOSIS BY TETRANDRINE, NEFERINE AND DAURICINE IN THE HUMAN MAMMARY MCF-7 MULTIDRUG RESISTANT CELLS

目的 观察粉防己碱、甲基莲心碱和蝙蝠葛碱与长春新碱合用对多药耐药肿瘤细胞诱导凋亡的作用。方法 采用荧光染料Hoechst 33342和碘化丙锭联染技术、流式细胞术和琼脂糖凝胶电泳技术 ,检测长春新碱及其与上述单体合用诱导耐药株瘤细胞MCF 7/Dox的凋亡程度。结果 单用长春新碱可引起约 10 %细胞凋亡 ,而长春新碱与粉防己碱、甲基莲心碱或蝙蝠葛碱合用明显地增加细胞凋亡的百分率 ,其中粉防己碱的作用最强。联合用药的细胞凋亡数显著地大于单用长春新碱的作用。结论 粉防己碱、甲基莲心碱和蝙蝠葛碱均有明显的增强长春新碱诱导人乳腺癌MCF

AIM To investigate the poteintation of vincristine induecd apoptosis by tetrandrine, neferine and dauricine isolated from Chinese medicinal plants in the human mammary MCF 7 multidrug resistant cells. METHODS The apoptotic cells were detected by fluorescent staining of a combination of Hoechst 33342 and propidium iodide (PI), flow cytometry and agarose electrophoresis. RESULTS The apoptotic cells induced by vincristine alone accounted for about 10% of all the cancer cells, while the percentage of apoptotic cells induced by a combination of vincristine with tetrandrine, neferine, or dauricine was found to be significantly higher than that by vincristine alone, and their reversal effects were positively correlated with the drug concentration and the exposure time. In addition, tetrandrine was shown to be the most potent in the reversal efficacy among the three compounds to be tested for apoptosis in vitro . CONCLUSION Tetrandrine, neferine and dauricine showed obvious potenitiation of vincristine induced apoptosis in the human mammary MCF 7 multidrug resistant cells.