钝顶螺旋藻糖缀合物SPPA-1的理化性质及活性的研究

PHYSICO CHEMICAL PROPERTIES AND BIOLOGICAL ACTIVITIES OF A GLYCOCONJUGATE SPPA 1 FROM SPIRULINA PLATENSIS

目的 研究螺旋藻多糖中的均一组分SPPA 1的分离方法、糖组成、分子量及生物活性。方法 采用丙酮分部沉淀 ,三氯乙酸去蛋白 ,再经SephadexG 75和CM SephadexC 5 0柱色谱得到SPPA 1,用气相色谱法测定单糖组成 ,GPC法测定分子量 ,IR和NMR法确定糖苷键类型。结果 经高压液相色谱和毛细管电泳鉴定为均一性组分。其总糖含量为 91 70 % ,N含量为 0 96 % ,分子量为 6 9 0 0× 10 4 Da。糖组成分析表明其由单一的葡萄糖组成 ,红外光谱、核磁共振1HNMR谱分析表明 ,其单糖类型为α 吡喃型。ESR研究表明SPPA 1具有清除O·2 自由基活性。结论 SPPA

AIM To isolate polysaccharide from Spirulina platensis and determine its sugar position, molecular weight and biological activities. METHODS Sephadex G 75 and CM Sephadex C 50 were used. The sugar position was analyzed by gas chromatography, the molecular weight was determined by GPC. The homogeneity of this glycoconjugate was determined by HPLC and CE. IR and NMR spectra were used to determine the glycosidic linkage. RESULTS SPPA 1 was a homogeneous glycoconjugate, its carbohydrate chain was composed of α glucopyranan, carbohydrate content is 91 70%, Nitrogen content was 0 96%, the molecular weight was 69 00×10 4, SPPA 1 could eliminate O · 2 radicals. CONCLUSION SPPA 1 was an anti oxidative glycoconjugate from Spirulina platensis .