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外源性雌激素及三苯氧胺对大鼠乳腺癌癌前期病变的作用 被引量:10

The effect of exogenous estrogen and tamoxifen on murine experimental precancerous breast disease
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摘要 目的 建立乳腺癌癌前期病变动物模型 ,探索外源性雌激素 (EE)及其拮抗剂的作用。方法 Wistar雌性大鼠 5 0只 ,对照组 12只 ;其余大鼠经胃管灌注 7,12 二甲基苯并蒽 (DMBA) ,8只用正常饮料 (DMBA组 ) ;15只饲料中加乙烯雌酚 (DMBA +DES组 ) ;15只饲料加三苯氧胺 (DMBA +TAM组 )。 3个月后检测乳腺增生、核仁形成区嗜银蛋白 (AgNOR)和DNA含量。结果 DMBA组和DMBA+DES组的乳腺组织增生率分别为 75 %及 93 33 % ;AgNOR计数为 3 71± 0 76及 5 6 0± 0 42 ;DNA含量为 88 5 9± 7 17及 137 75± 3 13,DMBA组及DMBA +DES组与对照组和DMBA +TAM组比较差异有显著意义 (P <0 0 1)。结论 一次性灌注DMBA可以建立乳腺癌癌前期病变动物模型 ;EE可加重DMBA诱导的乳腺癌癌前期病变 ; Objectiver [WT5”BZ] To establish murine breast precancerous model and evaluate the effect of exogenous estrogen and tamoxifen.Method 50 female Wistar rats were divided into contol group ( n =12), the other 38 received low dose carcinogen (DMBA) by gastrogavage, and then divided into DMBA group (common diet, n =8); DMBA+DES group(DES added chow, n =15); and DMBA+TAM group (TAM added chow, n =15). 3 months later, the status of breast hyperplasia, AgNOR and DNA content were measured.Results All indexs in control group and DMBA+TAM group were normal.However,in DMBA group and DMBA+DES group,the breast hyperplasia rate was 75%, 93.3%, AgNOR was 3.71±0.76, 5.60±0.42, DNA was 88.59±7.17, 137.75±3.13 respectively.Compared with control group and DMBA+TAM group, the differences of indexes in DMBA and in DMBA+DES group were significant ( P <0.01). [WT5”HZ]Conclusion [WT5”BZ] Low dosage DMBA administered by gastrogavage established breast precancerous model. Exogenous estrogen promotes the development of breast precancerous disease induced by DMBA, while TAM suppresses the canceration.
出处 《中华普通外科杂志》 CSCD 北大核心 2001年第2期110-112,共3页 Chinese Journal of General Surgery
关键词 乳腺肿瘤 外源性雌激素类 癌前病变 三苯氧胺 大鼠 Breast neoplasms Estrogens
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  • 1徐仁宝,生理科学进展,1983年,14卷,153页

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