Survivin原位杂交表达在宫颈癌及癌前病变诊断中的应用

Application of Survivin hybridization expression in situ in diagnosis of cervical cancer and cervical precancerous lesion

目的:探讨Survivin原位杂交表达在宫颈癌及癌前病变诊断中的应用效果。方法:宫颈癌患者45例作为观察组,另取宫颈上皮内瘤变45例作为对照Ⅰ组、正常宫颈组织45例作为对照Ⅱ组,所有研究对象给予Survivin原位杂交检测。结果:Survivin在对照Ⅱ组、对照Ⅰ组、观察组表达阳性率分别为2.2%、48.9%和84.4%,组间对比差异有统计学意义(P<0.05)。Survivin在宫颈癌Ⅰ期、Ⅱ期和Ⅲ期的阳性表达率对比差异有统计学意义(P<0.05)。Survivin在宫颈癌高分化组、中分化组及低分化组的阳性表达率对比差异也有统计学意义(P<0.05)。Survivin的表达与淋巴结转移无关(P>0.05)。结论:Suvrvini可在宫颈癌及癌前病变的发生发展中发挥作用,其Survivin表达程度与病期进展和不良组织学分级相一致,可作为预后不良的标志之一。

Objective： To explore the application effect of Survivin hybridization expression in situ in diagnosis of cervical cancer and cervical precancerous lesion. Methods： A total of 45 patients with cervical cancer, 45 patients with cervical intraepithelial neoplasia （ CIN）, and 45 patients with normal cervical tissue were selected as observation group, control I group, and control group, respective ly ; all the study objects received Survivin hybridization in situ. Results ： The positive expression rates of Survivin in control group, con trol group, and observation group were 2. 2%, 48.9%, and 84.4%, respectively, there was statistically significant difference among the three groups （ P 〈 0. 05 ） . There was statistically significant difference in the positive expression rate of Survivin in cervical cancer group a mong stage subgroup, stage subgroup, and stage subgroup （P 〈 0. 05） . There was statistically significant difference in the positive expression rate of Survivin in cervical cancer group among high differentiation subgroup, moderate differentiation subgroup, and low differen tiation subgroup （ P 〈 0. 05 ） . There was no correlation between Survivin expression and lymph node metastasis （ P 〉 0. 05 ） . Conclusion ： Suvrvini plays a key role in occurrence and development of cervical cancer and cervical precancerous lesion, and the degree of Survivin ex-pression is related to progression of the disease and adverse histological grade, which can be used as one of the signs of poor prognosis.