酶解制备褐藻胶寡糖及其产物的抗氧化活性分析

Alginate-derived oligosaccharides product by alginate lyase and detection of the antioxidant activity

以褐藻酸钠为底物,通过褐藻胶裂解酶酶解方法得到3种不同分子质量范围的褐藻胶寡糖组分(ADOA、ADO-B和ADO-C),对其抗氧化性活性进行了研究。结果表明:3种寡糖组分都有一定的抗氧化活性,且活性随着寡糖浓度的增加而加强,其中ADO-C的抗氧化活性最高。在DPPH·体系中,ADO-C的IC50值为0.53 mg/mL;在·OH体系中,ADO-C对羟基自由基的清除活性大于同浓度的抗坏血酸(P<0.05);还原力方面,在较低的浓度下,ADO-C表现出的还原力显著大于其余的2种(P<0.05)。实验表明,酶解得到的褐藻胶寡糖具有较强的抗氧化性,ADO-C(M W<8000)的抗氧化性最显著,即分子质量较小的寡糖有更强的抗氧化活性。

Alginate-derived oligosaccharides were obtained by enzymatic hydrolysis, and the antioxidant activities of oligosaecharides with different molecular weight range, ADO-A, ADO-B and ADO-C, were studied. The results showed that three kinds of oligosaeeharides showed certain antioxidant activities in a concentration dependent manner. Alginate oligosaecharides C showed the highest DPPH radical-scavenging activity, and the IC50 of ADO-C was 0.53mg/mL. Under the same concentration, ADO-C showed higher hydroxyl free radical-scavenging activity than Vc （ P 〈 0.05）. ADO-C also showed significant higher reducing power than ADO-A and ADO-B （P 〈 0.05）. These re- sults showed that the alginate oligosaccharides obtained by hydrolyzation of sodium alginateby alginatelyase had antiox- idant activities, wherein ADO-C （Mw 〈 8 000） showed the strongest activity, which indicated that the oligosacchaird- es with smaller molecular weight would have better antioxidant activities.