摘要
通过比较蒸汽爆破预处理前后灵芝制取的灵芝粗提物中功能性成分的提取率变化来反映蒸汽爆破技术对灵芝提取过程的影响,并对灵芝提取物进行安全性评价初步研究。以灵芝多糖,灵芝三萜提取率的变化来反映蒸汽爆破技术对灵芝粗提物的影响,采用急性毒性实验,30d饲养实验进行安全性初步评价。结果表明:1.5MPa,150s灵芝蒸汽爆处理后的多糖提取率是3h未处理组的10倍,蒸汽爆破2.1MPa,210s处理后灵芝粗提物中三萜的提取率增加为原来的1.95倍,本次实验初步评估表明蒸汽爆破法预处理制取的灵芝粗提物无明显的毒副作用及不良反应。
The effect of steam explosion on Ganoderma Lucidum extractive was evaluated by comparing the difference of functional components from treated and untreated Ganoderma Lucidum and the toxicity of extractive from Ganoderma Lucidum pretreatmented by steam explosion was evaluated. The evaluation indicators include extraction rates of Ganoderma Lucidum polysaccharide and Ganoderma Lucidum triterpene. The toxicity of Ganoderma Lucidum extract treated by steam explosion was evaluated by tests of acute toxicity and 30 days feeding test. The steam explosion for 150s at a steam pressure of 1.5MPa was effective pretreatment for the extraction of Ganoderma Lucidum polysaccharide and the polysaccharide extraction rate of treated sample was 10 times compared with the untreated sample (3h). The steam explosion for 210s at a steam pressure of 2.1MPa was effective pretreatment for the extraction of Ganoderma Lucidum triterpene and the triterpene extraction rate of treated sample was 1,95 times compared with the untreated sample. The in vivo mouse toxicity test showed that there is no obvious toxicity of extractive from Ganoderma Lucidum pretreatmented by steam explosion.
出处
《食品工业科技》
CAS
CSCD
北大核心
2014年第6期338-342,共5页
Science and Technology of Food Industry
基金
区域特产资源生态高值利用技术研究与产品开发(2011BAZ02169)
关键词
蒸汽爆破
灵芝多糖
灵芝三萜
毒理学
steam explosion
Ganoderma Lucidum polysaccharide
Ganoderma Lucidum triterpene
toxicology