草果甲醇溶出物对D-半乳糖致衰老小鼠的抗氧化作用机理研究

Study on mechanism of antioxidant effect of methanol extracts from tsao-ko(amomum tsao-ko crevost et lemaire) in aged mice induced by D-galactose

目的:了解明草果甲醇溶出物对D-半乳糖所致衰老小鼠的抗氧化功效。方法:采用D-半乳糖结合高脂饲料建立ICR小鼠衰老模型,高、低剂量组分别添加草果溶出物200、100mg/kg,考察血浆和肝组织中SOD、GSH、GSH-Px、MDA、8-ISO-PGF2α指标。结果:以模型为参照,血指标显示,草果组SOD活力极显著提高(p<0.01),草果组GSH含量均极显著提高(p<0.01),GSH-Px活力也极显著提高(p<0.01),草果组MDA含量均显示显著降低(p<0.05),高剂量组8-ISOPGF2α含量显著降低(p<0.05);肝指标显示,低剂量组SOD活力显著提高(p<0.05),高剂量组差异极显著(p<0.01),高剂量组GSH含量极显著提高(p<0.01),能极显著提高GSH-Px活力(p<0.01),极显著降低MDA含量(p<0.01);结论:草果甲醇溶出物能够显著提高小鼠SOD、GSH、GSH-Px指标,MDA和8-ISO-PGF2α含量显著下降。

Objective：To investigate the antioxidant effect of bioactive substances from tsao-ko in the aged ICR mice induced by D-galactose. Methods：Aged mice induced by high fat diet and D-galactose were feeding separately with different kinds of diets. High dose of tsao-ko was 200mg/kg,low dose of tsao-ko was 100mg/kg. The level of SOD,GSH,GSH-Px,MDA and 8-1SO-PGF2a in plasma,liver was investigated in aged mice in the experiment of the antioxidant activity in vivo. Results.Compared with the control group about the data of plasma,the activity of SOD in the aged mice conducted with tsao-ko was significantly improved （p〈0.01）,the content of GSH of mice deposed with tsao-ko was significantly improved （p〈0.01）,the activity of GSH-Px in the aged mice conducted with high dose of tsao-ko was significantly improved（p〈0.01 ）,the content of MDA in the aged mice conducted with tsao-ko was Iower（p〈0.05）,the content of 8-1SO-PGF2a in the aged mice conduced with high doses of tsao-ko was lower（p〈0.05）. Compared with the control group about the data of liver,the activity of SOD of mice conducted with low dose of tsao-ko was improved （p〈0.05） and significantly improved with high dose（p〈0.01 ）,the content of GSH in the aged mice conducted with high dose of tsao-ko was significantly improved（p〈0.01 ）,the activity of GSH-Px of mice conducted with tsao-ko was improved significantly（p〈0.01 ）,the content of MDA in the aged mice conducted with tsao-ko was lower significantly （p〈0.01）. Conclusion=The methanol extract of tsao-ko could increase the level of SOD,GSH,GSH-Px and reduce MDA and 8-1SO-PGF2α.