芩术四物汤中抗痛经有效成分的筛选

Selection of Anti-algomenorrhea Components of Qinshu Siwutang

目的:筛选芩术四物汤中抗痛经的有效部位与成分。方法:灌胃痛经模型小鼠(缩宫素诱发)芩术四物汤乙酸乙酯提取物(230、120 m/kg)、正丁醇提取物(1 040、520 m/kg)、水提取物(3 000、1 500 mg/kg),测定小鼠扭体反应次数,计算抑制率,以筛选有效提取物;灌胃痛经模型小鼠(缩宫素诱发)汉黄芩素(134、67 mg/kg)、黄芩素(144、72 mg/kg)、没食子酸(68.6、34.3 mg/kg)、黄芩苷(120、60 mg/kg),测定小鼠扭体反应次数,计算抑制率,以筛选有效成分;灌胃痛经模型小鼠[前列腺环素(PGF)2a诱发]没食子酸(68.6、34.3、17.2 mg/kg)、黄芩苷(120、60、30 mg/kg),测定小鼠扭体反应次数,计算抑制率。结果:230、120 mg/kg芩术四物汤乙酸乙酯提取物、68.6、34.3 mg/kg没食子酸、120 mg/kg黄芩苷可明显减少模型小鼠(缩宫素诱发)扭体次数,降低模型小鼠扭体抑制率(P<0.01或P<0.05);68.6、34.3 mg/kg没食子酸、120、60 mg/kg黄芩苷可明显减少模型小鼠(PGF2a诱发)扭体次数,降低模型小鼠扭体抑制率(P<0.01或P<0.05)。结论:芩术四物汤乙酸乙酯提取物中没食子酸、黄芩苷可能是芩术四物汤治疗痛经的有效成分。

OBJECTIVE： To select the effective components of Qinshu siwutang to algomenorrhea. METHODS： Algomenor- rhea model （induced by oxytocin） mice were given ethyl acetate extract （23, 12 mg/kg）, n-butyl alcohol extract （1 040, 520 mg/ kg） and water extract（3 000, 1 500 mg/kg） of Qinshu siwutang intragastrically; the times and inhibitory rate of writhing response in mice were determined to select effective extracts. Algomenorrhea model mice were given wogonin （134, 67 mg/kg）, baicalein （144, 72 mg/kg）, gallic acid （68：6, 34.3 mg/kg）, baicalin （120, 60 mg/kg） intragastrically; the inhibitory rate of writhing re- sponse in mice was determined to select effective components. Algomenorrhea model （induced by PGF2a） mice were given gallic acid （68.6, 34.3, 17.2 mg/kg） and baicalin （120, 60, 30 mg/kg） ; the inhibitory rate of writhing response in mice was determined. RESULTS：230,120 mg/kg ethyl acetate extract of Qinshu siwutang, 68.6, 34.3 mg/kg gallic acid and 120 mg/kg baicalin could sig- nificantly decrease the times and inhibitory rate of writhing response in mice; there was statistical significance （P〈0.01 or P〈 0.05）. 68.6, 34.3 mg/kg gallic acid and 120, 60 mg/kg baicalin could decrease the times and inhibitory rate of writhing response in model （induced by PGF2a） mice; there was statistical significance （P〈 0.01 or P〈 0.05）. CONCLUSIONS. The gallic acid and ba- icalin in ethyl acetate extract of Qinshu siwutang maybe the effective composition in the treatment of algomenorrhea.