妇血康颗粒的促排卵作用

Study on the Promoting Ovulation Effect of Fuxuekang Granules

目的:研究妇血康颗粒促排卵作用。方法:40只未成年雌性Wistar大鼠随机均分为正常对照(等容生理盐水)组、己烯雌酚(1 mg/kg)组与妇血康高、低剂量(0.4、0.1 g/kg)组,灌胃给药,每天1次,连续14 d。末次给药1 h后,检测大鼠血清黄体生成素(LH)、孕激素(P)、雌二醇(E2)和促卵泡激素(FSH)的分泌量;计算大鼠卵泡数;对大鼠子宫、卵巢组织进行病理切片观察。结果:与正常对照组比较,妇血康高、低剂量组大鼠血清LH活性增强,P含量减少,卵泡成熟总数增加,大鼠卵巢组织状态明显改善,差异有统计学意义(P<0.05),且大鼠子宫变化不大;妇血康高剂量组大鼠血清E2含量减少,差异有统计学意义(P<0.05);妇血康低剂量组大鼠血清FSH含量减少,差异有统计学意义(P<0.05)。结论:妇血康颗粒可调节大鼠下丘脑-垂体-卵巢(HPO)轴的功能,促进卵泡发育成熟和排卵。

OBJECTIVE： To study the promoting ovulation effect of Fuxuekang granules. METHODS：40 underage female SD rats were randomly divided into normal control group （constant volume of normal saline）, diethylstilbestrol groups （1 mg/kg）, and Fuxuekang low-dose and high-doe groups （0.1,0.4 g/kg）. The rats were given relevant medicines intragastrically once a day for con- secutive 14 days. 1 h after last administration, the contents of LH, progesterone （P）, estradiol （E2） and follicle stimulating hor- mone （FSH） in serum of rats were measured. The counting of mature follicle was calculated. The pathological section of uterus and ovary tissue were observed by light microscope. RESULTS： Compared with normal control group, the activities of LH, the count- ing of mature follicle in serum of rats increased in Fuxuekang low-dose and high-doe groups, while the content of P reduced; ovary tissue of rats were improved significantly; there were statistical significance（P〈0.05）. No obvious change was found in uterus. The content of E2 reduced in Fuxuekang high-dose group; there was statistical significance（P〈0.05）. The content of FSH reduced in Fuxuekang low-dose group; there was statistical significance （P〈0.05）. CONCLUSIONS： Fuxuekang granules could modulate hy- pothalamic-pituitary-gonadotrophic（HPO） function, and it can promote the follicular development and maturity, and then promote ovulation.