姜黄素对人宫颈癌Hela细胞增殖的抑制作用

Inhibitory Effects of Curcumin on the Growth of Human Cervical Carcinoma Hela Cells

目的:研究姜黄素对人宫颈癌Hela细胞生长的抑制作用。方法:姜黄经75%乙醇提取、纯化得姜黄素。MTT法检测姜黄素对体外培养Hela细胞增殖的抑制作用;倒置显微镜观察Hela细胞形态学变化;Western blot法检测抑癌基因p53蛋白的表达;流式细胞仪检测Hela细胞凋亡和周期分布。结果:姜黄素对Hela细胞生长24 h的最佳抑制质量浓度为116.68μg/ml,抑制率为63.20%;11.67、35.00、58.34μg/ml姜黄素培养24 h,人宫颈癌Hela细胞数目明显减少,细胞变圆、缩小、老化,核质发散,并与质量浓度呈正相关。29.17、145.85μg/ml姜黄素可增强p53蛋白的表达;11.67、35.00、58.34μg/ml姜黄素可促进人宫颈癌Hela细胞凋亡,并与质量浓度呈正相关,G0/G1期细胞和S期细胞逐渐减少,G2/M期细胞逐渐增多。结论:姜黄素对人宫颈癌Hela细胞增殖有明显抑制作用,并能促进其凋亡和p53的表达,阻滞Hela细胞的G2/M期。

OBJECTIVE： To investigate the inhibitory effect of curcumin on the growth of human cervical carcinoma Hela cells. METHODS： Curcumin was extracted and purified by 75 % ethanol. Hela cells were cultured in vitro, and inhibitory effects of curcumin on the growth of Hela cells were measured with MTT method to explore optimal inhibitory concentrations; the morpholog- ic change of human cervical carcinoma Hela cells was observed by inverted microscope; the expression of p53 protein in Hela cell was observed by Western blot method. In addition, cell apoptosis and cell cycle were inspected by flow cytometry. RESULTS： The optimal inhibitory concentration of curcumin to Hela cells in 24 h was 116.68 μg/ml, with inhibitory ratio of 63.20%. After treated with 11.67,35.00,58.34 μg/ml curcumin for 24 h, the number of human cervical carcinoma Hela cells decreased significantly, cell rounding, shrinking and aging was in concentration-dependant manner. The p53 protein expression was increased after treated with 29.17,145.85 μg/ml curcumin; 11.67,35.00,58.34μg/ml curcumin could promote the apoptosis of human cervical carcinoma Hela cells, in concentration-dependant manner. With the increase of curcumin concentration, the number of cells in G0/G1 phase and S phase were decreased while the number of cells in G2/M phase was increased. CONCLUSIONS： Curcumin has obvious inhibitory effect on human cervical carcinoma Hela cells, promote the apoptosis of Hela cells and the expression of p53 protein. It also pre- vents the cells in G2/M phase.