辛伐他汀对人鼻息肉成纤维细胞增殖能力和胶原合成的影响

Effect of simvastatin on the proliferation and collagen synthesis of human nasal polyps fibroblasts

目的体外研究辛伐他汀(SIM)对人鼻息肉成纤维细胞(NPFS)增殖能力及胶原分泌的影响。方法用不同浓度SIM与体外培养的人NPFS作用不同的时间,用CCK-8比色法检测细胞的增殖,ELISA法测定细胞培养上清液Ⅲ型胶原的含量。结果 SIM(0.1、1、10、100μmol/L)对人NPFS生长情况的影响随着作用时间(48h、96h、144h)延长而出现不同的结果。作用48h各种浓度SIM均不能抑制人NPFS生长。而作用时间的延长(96h、144h),SIM(1、10、100μmol/L)均能抑制人NPFS生长,CCK-8比色法Am值呈递减的趋势,与对照组相比,差异均具有统计学意义(均P<0.05)。各种浓度辛伐他汀组作用144h均能使人NPFS培养上清液中的Ⅲ型胶原的含量呈逐渐递减的趋势,与对照组相比,差异具有统计学意义(均P<0.01)。结论一定浓度的降脂药物SIM作用一定的时间能抑制人NPFS增殖和胶原分泌,可能对阻止上呼吸道组织重塑、预防鼻息肉的复发有一定的潜在临床应用价值。

Objective To investigate the effect of simvastatin on the proliferation, collagen excretion of human nasal pol- yps fibroblasts （NPFS）. Methods Human NPFS were isolated and cultured. Nasal polyps fibroblast proliferation was meas- ured by CCK-8 assay. Collagen III in the conditioned media was determined by ELISA. Results SIM （0.1, 1, 10, 100 μmol/ L） on the impact of the growth of human NPFS with different time （48 h, 96 h, 144 h） came up with different results. 48 h with various concentrations of SIM could not inhibit the growth of NPFS. Along with the extension of time （96 h, 144 h） with SIM （1, 10, 100 μmol/L）, SIM could inhibit the NPFS growth. CCK-8 colorimetric Am values showed decreasing trend, with the control compared, the difference was statistically significant （all P〈0.05）. 144 h with various concentrations of simv- astatin could make NPFS supernatants of type llI collagen gradually decreasing, compared with the control group, the difference was statistically significant （all P〈0.01）. Conclusion Simvastatin inhibits the proliferation of cultured human nasal polyps and the collagen excretion systhesis effectively. Further study of statins as the potential therapeutic medicines for nasal polyps fibro- blasts is necessary.