一起暴发流行的无菌性脑炎的病原分析

Detection and etiological analysis of an aseptic meningitis outbreak

目的对一起暴发流行的无菌性脑炎进行病原分析。方法用荧光PCR法对14份咽拭子标本进行核酸定性检测,细胞培养分离得到病毒后进行微量细胞中和试验,并对病毒VP1区3’段核酸测序以鉴定病毒。结果 14份咽拭子标本中,甲型/乙型流感病毒、肠道病毒71型(EV71)和柯萨奇病毒A16型(Cox A16型)均为阴性,其中8份标本肠道病毒通用型为阳性。从该8份标本从中分离到3株疑似肠道病毒,经微量细胞中和试验及病毒VP1区3’段核酸测序鉴定,均为埃可病毒30(ECHO30)型肠道病毒。结论本次暴发的无菌性脑炎由ECHO30型肠道病毒引起。

Objective To investigate the etiology of an aseptic meningitis outbreak at a primary school in Wuxi city. Methods Real-time RT-PCR was used to screen for viruses of interest among throat swab samples. After inoculation with en- terovirus-positive swabs, virus strains were isolated from Vero or RD cells. These strains Were identified by the serum neutral- ization test and by sequencing the 3＇ ends of their VP1 regions. Results Among the 14 throat swabs, none was positive for in- fluenza virus A/B, enterovirus 71 （EV71）, or coxsackievirus A16 （Cox A16）. Eight of the 14 swabs were positive for enterovir- uses, from which three virus strains were isolated. Based on the serum neutralization test and their VP1 region sequences, those three virus strains were identified as ECHO30 enterovirus. Conclusion ECHO30 was the major etiological agent responsible for the outbreak of aseptic meningitis at the primary school in Wuxi city.