自然杀伤细胞内穿孔素与干扰素-γ在毛细支气管炎中的变化

The change of perform-protein and IFN-γ in nature killer cells of children with capillary bronchiolitis

目的探讨外周血自然杀伤细胞(NK)内穿孔素(Perform-protein,PFP)、干扰素-γ(IFN-γ)在毛细支气管炎中的变化。方法选择呼吸道合胞病毒感染毛细支气管炎(RSVB)患儿27例(毛支组),流感病毒性肺炎(IVP)患儿25例(流感肺炎组),健康婴幼儿20例(健康组)。取外周静脉血标本,采用密度梯度离心法分离获得外周血单核细胞,流式细胞仪检测NK细胞数量,免疫磁珠分选技术纯化NK细胞,采用标准刺激剂、离子霉素(PMA)与佛波酯(Ion)刺激NK细胞,流式细胞技术分析NK细胞内PFP、IFN-γ的表达情况。结果①毛支组、流感肺炎组较健康组NK细胞数量减少,组间比较差异均有统计学意义(P<0.05)。②在PMA+Ion下刺激NK细胞,与健康组比较,毛支组NK细胞内IFN-γ表达升高,差异具有统计学意义(P<0.05);毛支组、流感肺炎组NK细胞内PFP表达降低,与健康组比较差异均有统计学意义(P<0.05),而毛支组与流感肺炎组比较差异无统计学意义(P>0.05)。结论 NK细胞数量及其细胞内的穿孔素及IFN-γ的变化参与毛细支气管炎的发病。

Objective To explore change of Perform-protein and IFN-y in nature killer cells of children with capillary bronchiolitis. Methods 27 children with respiratory syncytial virus bronchiolitis （RSVB） and 25 children with influenza viral pneumonia （IVP） and 20 healthy children were recruited. Peripheral blood mononuclear cells were collected by the way of density gradient centrifugation method from blood samples. The percentage of nature killer cells were measured by flow cytometry. NK cells were isolated with immunomagnetic beads and stimulated by standard stimulus （PMA＋ionomcycin） respectively. The levels of PFP and IFN-y were measured by flow cytometry. Results （1）Compared with the control group, the percentage of nature killer cells in RSVB group and IVP group were decreased （P〈0.05）. （2）Under being cultured with PMA＋Ion, compared with the control group, the level of IFN-y in nature killer cells expressed higher that RSVB group （P〈0.05） ; the level of PFP showed lower in RSVB group and IVP group, there was statistical significance （P〈0.05） ;there were no statistical significance of the levels of PFP between RSVB group and IVP group （P〉0.05）. Conclusion The changes of the percentage and the levels of PFP and IFN-y in nature killer cells may contribute to capillary bronchiolitis of children.